Secondary caries development and the role of a matrix metalloproteinase inhibitor: A clinical in situ study

• Published in: Journal of dentistry 2018-04, Vol.71, p.49-53
• Main Authors: Maske, T.T., Kuper, N.K., Hollanders, A.C.C., Bronkhorst, E.M., Cenci, M.S., Huysmans, M.C.D.N.J.M.
• Format: Article
• Language: English
• Subjects: Adhesives; Adult; Biofilms; Bond strength; Chlorhexidine - pharmacology; Composite Resins; Dental Bonding; Dental caries; Dental Caries - prevention & control; Dental Cements; Dental Occlusion; Dental Restoration, Permanent; Dental restorative materials; Dentin; Dentin - drug effects; Dentin - pathology; Dentistry; Female; Humans; in situ; Male; Materials Testing; Matrix metalloproteinase; Matrix Metalloproteinase Inhibitors - pharmacology; Metalloproteinase; Mmp; Resin Cements; Secondary caries; Studies; Wall lesions
• ISSN: 0300-5712; 1879-176X
• DOI: 10.1016/j.jdent.2018.01.011

This in situ study aimed to investigate whether the dentin treatment with MMPs inhibitor (CHX 2%) could influence the development of secondary caries wall lesions in different dentin-composite interfaces. For 21 days, 15 volunteers wore a modified-occlusal splint loaded with dentin-composite samples treated or not with CHX and restored according 4 different interface conditions: Bonding (B = samples restored with complete adhesive procedure), no bonding (NB = restored with composite resin without adhesive procedure), 100 μm (no adhesive procedure and with intentional gap) and 100 μm + B (adhesive material on composite side and intentional gap). Eight times per day, the splint with samples was dipped in a 20% sucrose solution for 10 min. Before and after caries development, samples were imaged with T-WIM and lesion depth (LD) and mineral loss (ML) were calculated. Linear mixed effect analysis showed that dentin treatment with CHX did not significantly affect the caries lesion progression (LD and ML; p ≤ 0.797). Dentin wall lesions were observed in the 100 μm and 100 μm + B groups independently of MMP inhibitor treatment. The treatment of dentin with MMP inhibitor was not able to slow down the secondary caries wall lesion development in this in situ study. The dentin treatment with 2% CHX did not prevent secondary caries wall lesion initiation.