Sensitivity evaluation of the Daphtoxkit and Thamnotoxkit microbiotests on blind samples

• Published in: Journal of applied toxicology 2004-09, Vol.24 (5), p.323-326
• Main Author: Toeroekne, A
• Format: Article
• Language: English
• Subjects: acute toxicity; Animals; Biological Assay - methods; Bioterrorism; Cladocera; Cost Control; Daphnia; Daphnia magna; Daphtoxkit; Environmental Monitoring - economics; Environmental Monitoring - methods; Freshwater; Humans; microbiotests; Sensitivity and Specificity; Thamnocephalus platyurus; Thamnotoxkit; Toxicity Tests - economics; Toxicity Tests - methods; Toxins, Biological - analysis; Water Pollutants - toxicity
• ISSN: 0260-437X; 1099-1263
• DOI: 10.1002/jat.1018

At the Oregon State University Workshop we utilized two ‘culture/maintenance‐free’ microbiotests, the Thamnotoxkit F™ with the anostracan crustacean Thamnocephalus platyurus and the Daphtoxkit F™ magna with the cladoceran crustacean Daphnia magna, to determine the toxicity of water samples in a blind format. The Thamnotoxkit was applied to 7 samples and the Daphtoxkit to 12 samples. The chemical composition of the water samples to which the Toxkit microbiotests had been applied was disclosed a posteriori by the organizers and appeared to contain chlordimeform, colchicine, phosdrin, mercuric chloride, sodium arsenite, metham sodium, sodium cyanide, trimethylol propane phosphate, p‐chlorophenol and a natural sediment sample containing mercury. Three of the water samples were blanks that had not been spiked with chemicals. No false positives were obtained with the two Toxkits and all the toxic waters were earmarked as such by the microbiotests, except trimethylol propane phosphate, which was not found to induce acute effects in Daphnia magna in the non‐diluted water sample containing 100 mg l−1 of this compound. Copyright © 2004 John Wiley & Sons, Ltd.