Protein fingerprinting profiles in different strains of Aeromonas hydrophila isolated from diseased freshwater fish

Aeromonas hydrophila(Ah) strains isolated from diseased fish in India were studied for protein profiling using the SDS-PAGE protein fingerprinting profile pattern of whole cells of 12 local strains of A. hydrophilaand one reference strain (MTCC 646). Variability among the strains was observed. The a...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2005-06, Vol.21 (4), p.587-591
Main Authors: Das, B.K, Samal, S.K, Samantaray, B.R, Meher, P.K
Format: Article
Language:English
Subjects:
Aeromonas hydrophila
Bacteria
bacterial infections
bacterial proteins
Biological and medical sciences
Biotechnology
catfish
Fish
Fish diseases
Freshwater
Freshwater fish
Fundamental and applied biological sciences. Psychology
goldfish
Proteins
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Summary:Aeromonas hydrophila(Ah) strains isolated from diseased fish in India were studied for protein profiling using the SDS-PAGE protein fingerprinting profile pattern of whole cells of 12 local strains of A. hydrophilaand one reference strain (MTCC 646). Variability among the strains was observed. The average similarity between the 12 strains of A. hydrophila ranged from 0.272 to 0.916. Proteins with molecular mass of 55.6 and 14.67 kDa in Ah1, Ah2 and Ah3, 28.5 and 27.9 kDa in Ah4, Ah5 and Ah6, 21.4 and 19.5 kDa in Ah7, Ah8, Ah9 and 72.9, 91.5 and 71.3 kDa in Ah10, Ah11 and Ah12 were common. The protein polypeptide bands from 19.5 to 86.2 kDa were common in both local strains and reference strain of A. hydrophila. The protein fingerprinting study showed that there is genetic similarity between strains of A. hydrophila and reference strain (MTCC 646). These protein markers may be useful for further strain differentiation in epidemiological study.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-004-3909-2