Microsatellite Analysis of Three Phylogenetic Species of Paracoccidioides brasiliensis

Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, the existence of three different phylogenetic species (S1, PS2, and PS3) of P. brasiliensis was demonstrated. Despite being genetically isolated, all thre...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-06, Vol.44 (6), p.2153-2157
Main Authors: Matute, Daniel R, Sepulveda, Victoria E, Quesada, Lina M, Goldman, Gustavo H, Taylor, John W, Restrepo, Angela, McEwen, Juan G
Format: Article
Language:English
Subjects:
Alleles
Gene Frequency
Genetic Markers - genetics
Humans
Microsatellite Repeats - genetics
Molecular Sequence Data
Mycological Typing Techniques
Mycology
Paracoccidioides - classification
Paracoccidioides - genetics
Paracoccidioides brasiliensis
Phylogeny
Sequence Analysis, DNA
Species Specificity
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Summary:Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, the existence of three different phylogenetic species (S1, PS2, and PS3) of P. brasiliensis was demonstrated. Despite being genetically isolated, all three species were capable of inducing disease in both humans and animals, although lower virulence has been found with the PS2 species. The available molecular methods developed to characterize and type strains have not been useful for assigning isolates to the described species, creating the need for molecular markers capable of distinguishing genetically isolated groups. Here, we describe a PCR and sequencing-based microsatellite marker system that is stable, easy to assay, adaptable to large series of isolates, and discriminatory enough to be used as a typing system in identifying the three proposed species of P. brasiliensis. In addition, this system provides an unambiguous tool for strain discrimination between two (S1 and PS2) of the three phylogenetic species.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.02540-05