Evaluation of methods for the isolation, detection and quantification of cyanobacterial hepatotoxins

Cyanobacterial hepatotoxins such as microcystins and nodularins have been responsible for the poisoning of both animals and humans who ingest or come into contact with toxic blooms. They are extremely stable in water due to their stable chemical structure and can tolerate radical changes in water ch...

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Bibliographic Details
Published in:Aquatic toxicology 2006-07, Vol.78 (4), p.382-397
Main Authors: Msagati, Titus A.M., Siame, Bupe A., Shushu, Deborah D.
Format: Article
Language:English
Subjects:
algal blooms
analytical methods
Animals
Bacterial Toxins - analysis
Bacterial Toxins - isolation & purification
Bacterial Toxins - poisoning
Bacterial Toxins - toxicity
bioassays
chemical analysis
chemical structure
Chromatography, High Pressure Liquid
Cyanobacteria
Cyanobacteria - chemistry
Cyanobacteria - metabolism
cyanobacterial toxins
detection
Electrophoresis, Capillary
Environmental Exposure - adverse effects
Enzyme-Linked Immunosorbent Assay
Eutrophication
extraction
Gas Chromatography-Mass Spectrometry
Hepatatoxins
hepatotoxins
Humans
literature reviews
Liver - drug effects
Liver - metabolism
Marine Toxins - analysis
Marine Toxins - isolation & purification
Marine Toxins - poisoning
Marine Toxins - toxicity
microbial growth
Microcystins
Nodularins
Nuclear Magnetic Resonance, Biomolecular
Peptides, Cyclic - analysis
Peptides, Cyclic - isolation & purification
physiological response
public health
quantitative analysis
sampling
toxicity
Water Microbiology
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Description
Summary:Cyanobacterial hepatotoxins such as microcystins and nodularins have been responsible for the poisoning of both animals and humans who ingest or come into contact with toxic blooms. They are extremely stable in water due to their stable chemical structure and can tolerate radical changes in water chemistry, including pH and salinity. Different methods for the extraction and detection of these compounds have been reported. Extraction methods utilizing both aqueous and organic solvent systems have been reported. The detection methods ranging from immunological or biochemical assays such as enzyme linked immunosorbent assays (ELISA) and enzyme activity assays, to chemicals methods such as high performance liquid chromatography (HPLC) and more sophisticated liquid chromatography–mass spectrometry (LC–MS) have been documented as well. We review some important aspects of cyanobacterial hepatotoxins and methods of analysis for these toxins.
ISSN:0166-445X
1879-1514
DOI:10.1016/j.aquatox.2006.03.011