Modeling the growth of Corynebacterium glutamicum under product inhibition in l-glutamic acid fermentation

In the fermentation of l-glutamic acid by Corynebacterium glutamicum, the growth inhibition by the substrate (glucose) at higher concentrations, and by the product at almost all concentrations seem to occur. In order to identify the range of concentrations for substrate limitation/inhibition, the ex...

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Bibliographic Details
Published in:Biochemical engineering journal 2005-09, Vol.25 (2), p.173-178
Main Authors: Khan, Noor Salam, Mishra, Indra Mani, Singh, R.P., Prasad, Basheshwer
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Corynebacterium glutamicum
Fermentation
Fundamental and applied biological sciences. Psychology
l-Glutamic acid
Modeling
Monod's equation
Product inhibition
Substrate limitation
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Summary:In the fermentation of l-glutamic acid by Corynebacterium glutamicum, the growth inhibition by the substrate (glucose) at higher concentrations, and by the product at almost all concentrations seem to occur. In order to identify the range of concentrations for substrate limitation/inhibition, the experiments were conducted separately with different initial glucose concentrations. Proof of growth inhibition by the product was established by analyzing the data obtained from the time course of batch fermentation. Based on the experimental observations, a product inhibition model has been developed by modifying the Monod's kinetic equation for cell growth. This model simulates the growth satisfactorily. The same model is also able to describe the experimental data for growth of C. glutamicum obtained from different investigators.
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2005.01.025