Application of denaturing gradient gel electrophoresis (DGGE) analysis to evaluate acetic acid bacteria in traditional balsamic vinegar

Acetic acid bacteria (AAB) are fastidious micro-organisms to isolate and cultivate despite of the great number of growth media available. Moreover, conventional techniques used to study AAB populations are time consuming and not completely reliable. In this study, we tested the usefulness of the pol...

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Bibliographic Details
Published in:Food microbiology 2006-12, Vol.23 (8), p.809-813
Main Authors: De Vero, Luciana, Gala, Elisabetta, Gullo, Maria, Solieri, Lisa, Landi, Sara, Giudici, Paolo
Format: Article
Language:English
Subjects:
Acetic Acid
Acetic acid bacteria
Biological and medical sciences
biotransformation
Consumer Product Safety
DGGE
DNA, Bacterial - analysis
Electrophoresis, Agar Gel - methods
Fermented food industries
Food Contamination - analysis
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
gel electrophoresis
grape must
Humans
isolation
Lactobacillus - isolation & purification
microbial colonization
molecular systematics
oak barrels
PCR
polymerase chain reaction
ribotypes
serotypes
Species Specificity
starter cultures
Traditional balsamic vinegar
traditional foods
vinegars
wine aging
Wines and vinegars
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Summary:Acetic acid bacteria (AAB) are fastidious micro-organisms to isolate and cultivate despite of the great number of growth media available. Moreover, conventional techniques used to study AAB populations are time consuming and not completely reliable. In this study, we tested the usefulness of the polymerase chain reaction-denaturing gradient gel electophoresis (PCR-DGGE) as a rapid and cost effective method for the screening of AAB in traditional balsamic vinegar (TBV). DGGE analysis was applied to 19 AAB strains isolated by agar plating from three different samples of TBV. DGGE was also used for the analysis of PCR products obtained from DNA extracted directly from the TBV samples. A tentative species identification was achieved comparing the PCR-DGGE patterns of the isolated strains and the TBV samples to those of 15 AAB reference strains. The results support that DGGE is functional to monitor vinegar's AAB population.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2006.01.006