A novel and in situ technique for the quantitative detection of MTBE and benzene degrading bacteria in contaminated matrices

A novel and in situ technique is presented here as a better alternative to culture-dependent and PCR-based techniques for the quantitative detection of predominant bacterial species involved in the bioremediation of contaminants. It allowed rapid, specific and in situ identification of Biosep®-immob...

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Bibliographic Details
Published in:Journal of microbiological methods 2007-02, Vol.68 (2), p.437-441
Main Authors: Biggerstaff, J.P., Le Puil, M., Weidow, B.L., Leblanc-Gridley, J., Jennings, E., Busch-Harris, J., Sublette, K.L., White, D.C., Alberte, R.S.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Benzene - metabolism
Biodegradation, Environmental
Biological and medical sciences
Bioremediation
Deltaproteobacteria - isolation & purification
Deltaproteobacteria - metabolism
Eubacteria
FISH
Fuel oxygenate
Fundamental and applied biological sciences. Psychology
In Situ Hybridization, Fluorescence - methods
Methyl Ethers - analysis
Methyl Ethers - metabolism
Microbiology
MTBE
Soil Microbiology
Soil Pollutants - metabolism
Spectral imaging
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Summary:A novel and in situ technique is presented here as a better alternative to culture-dependent and PCR-based techniques for the quantitative detection of predominant bacterial species involved in the bioremediation of contaminants. It allowed rapid, specific and in situ identification of Biosep®-immobilized eubacteria from MTBE- and benzene-contaminated matrices.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2006.09.016