Impact of azadirachtin, an insecticidal allelochemical from neem on soil microflora, enzyme and respiratory activities

• Published in: Bioresource technology 2007-11, Vol.98 (16), p.3154-3158
• Main Authors: Gopal, Murali, Gupta, Alka, Arunachalam, V., Magu, S.P.
• Format: Article
• Language: English
• Subjects: allelochemicals; Azadirachta - chemistry; Azadirachta indica; Azadirachtin; Azotobacter; Bacteria - drug effects; Bacteria - enzymology; Bacterial Proteins - metabolism; Biological and medical sciences; Enzymes; Fundamental and applied biological sciences. Psychology; Fungal Proteins - metabolism; Fungi - drug effects; Fungi - enzymology; Insecticides - pharmacology; Limonins - pharmacology; Microflora; Neem; Oxidoreductases - metabolism; Phosphoric Monoester Hydrolases - metabolism; Soil; Soil Microbiology; soil microorganisms; Soil Pollutants - pharmacology
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2006.10.010

The effect of 10% azadirachtin granules (alcoholic extract of neem seed kernel mixed with China clay) was studied on the population of bacteria, actinomycetes, fungi, Azotobacter and nitrifying bacteria; soil dehydrogenase, phosphatase and respiratory activities on 0, 15th, 30th, 60th and 90th days after application in sandy loam soil collected from the fields. It was observed that baring the Azotobacter sp., azadirachtin at all the doses exerted a suppressive effect on the rest of the microbial communities and enzyme activities in the initial 15 day period. The population of bacteria, actinomycetes besides phosphatase and respiratory activities recovered after 60th day and subsequently increased significantly. The fungi and nitrifiers were most sensitive groups as their numbers were reduced significantly throughout the studies. The two times and five times recommended dose of azadirachtin had very high biocidal effects on the soil microorganisms and its activities. However, analysis of the data by the Shannon Weaver index showed that azadirachtin reduces both the form and functional microbial diversity at all doses.