Detection of harmful cyanobacteria and their toxins by both PCR amplification and LC-MS during a bloom event

We briefly report here the occurrence of toxic blooms in the eutrophic reservoir Billings, São Paulo city, Brazil. Water samples were collected in May 2004, during a cyanobacterial bloom. The presence of toxic species was confirmed by using PCR amplifications of a fragment region of genes encoding m...

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Bibliographic Details
Published in:Toxicon (Oxford) 2006-09, Vol.48 (3), p.239-245
Main Authors: Anjos, Fabyana Maria dos, Bittencourt-Oliveira, Maria do Carmo, Zajac, Meron Petro, Hiller, Susann, Christian, Bernd, Erler, Katrin, Luckas, Bernd, Pinto, Ernani
Format: Article
Language:English
Subjects:
Bacterial Toxins - analysis
Bacteriology
Base Sequence
Billings reservoir
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cyanobacteria
Cyanobacteria - genetics
Cyanobacteria - growth & development
Cyanobacteria - isolation & purification
Cyanophyta
DNA Primers
Electrophoresis, Agar Gel
Fundamental and applied biological sciences. Psychology
Gonyautoxin
Harmful algae bloom
Liquid chromatography-mass spectrometry
Mass Spectrometry - methods
Medical sciences
Microbiology
Microcystin
Microcystis
mycB
Neosaxitoxin
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
PCR
Plant poisons toxicology
Polymerase Chain Reaction - methods
Saxitoxin
Toxicology
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Summary:We briefly report here the occurrence of toxic blooms in the eutrophic reservoir Billings, São Paulo city, Brazil. Water samples were collected in May 2004, during a cyanobacterial bloom. The presence of toxic species was confirmed by using PCR amplifications of a fragment region of genes encoding microcystin synthetase- mcyB. The determination of toxins was performed by liquid chromatography coupled with mass spectrometry (LC-MS). LC-MS analyses of the toxins from the bloom revealed variants of microcystins (MC), such as MC-LR, MC-RR and MC-YR. HPLC-FLD was used to determine the paralytic shellfish poisoning (PSP) saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins 2 (GTX2) and 3 (GTX3). GTX2, GTX3 and NEO were detected for the first time in a natural sample from Billings reservoir. These results are a contribution to the knowledge of the biogeography of toxic cyanobacteria and their toxins, specifically in São Paulo.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2006.05.006