Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria

We developed a novel single-stage autotrophic nitrogen-removal process comprised of two composite immobilized biomass layers-one of nitrifying bacteria and one of sulfur-denitrifying bacteria and elemental sulfur-in a Fe-Ni fibrous slag matrix. Nitrification and consumption of dissolved oxygen occur...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 2005-07, Vol.68 (1), p.124-130
Main Authors: AOI, Y, SHIRAMASA, Y, KAKIMOTO, E, TSUNEDA, S, HIRATA, A, NAGAMUNE, T
Format: Article
Language:English
Subjects:
Aerobiosis
Ammonia
Bacteria
Bacteria - metabolism
Biological and medical sciences
Biological treatment of waters
Biomass
Bioreactors
Biotechnology
Dissolved oxygen
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Microbiology
Nitrification
Nitrogen
Nitrogen - metabolism
Nitrogen removal
Oxidation-Reduction
Quaternary Ammonium Compounds - metabolism
Reactors
Sewage - microbiology
Slag
Sulfur
Sulfur - metabolism
Time Factors
Wastewater treatment
Water treatment
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We developed a novel single-stage autotrophic nitrogen-removal process comprised of two composite immobilized biomass layers-one of nitrifying bacteria and one of sulfur-denitrifying bacteria and elemental sulfur-in a Fe-Ni fibrous slag matrix. Nitrification and consumption of dissolved oxygen occurred in the outer part and sulfur denitrification in the anoxic inner part of the composite matrix, thus realizing autotrophic nitrogen removal in a single reactor. The complete conversion of ammonia into N2 in a single reactor was demonstrated in both batch-mode incubation and continuous-feed operation. The spatial profiles of the ammonia-oxidizing bacteria and denitrifying bacteria were evaluated by real-time PCR, targeting their functional genes, and stratification of these two types was observed in the matrix after several months of incubation. This process does not require any specific reactor type or conditions and thus has the potential to be applied to many different wastewater treatment processes due to its simplicity in both operation and construction.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-005-1910-9