Alginate production by Pseudomonas mendocina in a stirred draft fermenter

In this study alginate production by Pseudomonas mendocina in a laboratory-scale fermenter was investigated. In the experiments the effect of temperature (25-31°C) and agitation (500-620 rev min-¹) at a constant air flow of 10 v/v/h were evaluated in relation to the rate of glucose bioconversion to...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2007-05, Vol.23 (5), p.691-695
Main Authors: Müller, José Miguel, Monte Alegre, Ranulfo
Format: Article
Language:English
Subjects:
Agitation
Air flow
Alginate
Alginates
Alginic acid
Azotobacter
Azotobacter vinelandii
Bacterial alginate
Bioconversion
Biological and medical sciences
Biotechnology
Draft tubes
Fermentation
Fundamental and applied biological sciences. Psychology
Production
Pseudomonas
Pseudomonas mendocina
Response surface methodology
Rheological properties
Temperature effects
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Summary:In this study alginate production by Pseudomonas mendocina in a laboratory-scale fermenter was investigated. In the experiments the effect of temperature (25-31°C) and agitation (500-620 rev min-¹) at a constant air flow of 10 v/v/h were evaluated in relation to the rate of glucose bioconversion to alginate using response surface methodology (RSM). The fermenter configuration was also adapted to a system with a screw mixer and draft tube, due to the change in rheological characteristics of the fermentation broth. The adjusted model indicates a temperature of 29.1°C and agitation of 553 rev min-¹ for optimum alginate synthesis. In this fermentation system a Y p/s of 44.8% was achieved. The alginate synthesized by P. mendocina showed a partially acetylated pattern as previously reported for alginates obtained from other Pseudomonas spp and Azotobacter vinelandii.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-006-9285-3