Marine actinomycetes as bioremediators in Penaeus monodon rearing system

• Published in: Fish & shellfish immunology 2018-04, Vol.75, p.231-242
• Main Authors: Babu, Divya T., Archana, K., Kachiprath, Bhavya, Solomon, Solly, Jayanath, G., Singh, I.S. Bright, Philip, Rosamma
• Format: Article
• Language: English
• Subjects: Biogranules; Bioremediation; Marine actinomycetes; Nocardiopsis alba; Penaeus monodon; Streptomyces
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2018.01.037

Actinomycetes (277 Nos) isolated from marine environment and shrimp culture pond sediments were tested for hydrolytic enzyme production and biogranulation property. Potential isolates were screened for their efficacy in bioremediation of shrimp culture system. Based on the BOD reduction efficiency and water quality parameters, five actinomycete isolates viz., Streptomyces coelicoflavus (A6), Streptomyces diastaticus (A44), Nocardiopsis alba (A55), Streptomyces parvus (A56) and Streptomyces champavatii (R32) were subjected for tertiary screening in Penaeus monodon larval rearing system and the animals were challenged with white spot syndrome virus (WSSV). The bioremediating effect of actinomycete treatments were assessed by analysing the expression profile of five antimicrobial peptide (AMP) genes viz., anti-lipopolysaccharide factor (ALF), crustin-2, crustin-3, penaeidin-3 and penaeidin-5 and eight immune genes viz., alpha-2-macroglobulin (α-2-M), astakine, glutathione-S-transferase, haemocyanin, peroxinectin, pmCathepsinC, prophenol oxidase (proPO) and Rab-7. Expression of eight WSSV genes viz., DNA polymerase, endonuclease, protein kinase, immediate early gene, latency related gene, ribonucleotide reductase, thymidine kinase and VP28 were also analyzed to detect the presence and intensity of viral infection in the experimental animals post-challenge. Theapplication of consortia (1 g/5 L water) yields better results in terms of significant reduction in BOD of shrimp rearing system showing the bioremediation potential of the marine actinomycete strains. The application of marine actinomycetes viz., Streptomyces coelicoflavus (A6), Streptomyces diastaticus (A44), Nocardiopsis alba (A55), Streptomyces parvus (A56) and Streptomyces champavatii (R32) in granulated form were found to be potential bioremediators in shrimp rearing system. •Significant reduction in organic matter could be effected by marine actinomycetes in P. monodon larval rearing system.•Combination of Streptomyces and Nocardiopsis strains can be effectively used as bioremediators in shrimp hatchery.•Granulated forms of actinomycetes enable effective broadcasting into the shrimp culture system.