Morphological and ssrDNA sequence based molecular characterization of a novel Thelohanellus species (Myxosporea: Myxobolidae) infecting the fins of Goldfish, Carassius auratus L. with special reference to its histopathological alteration

• Published in: Acta tropica 2018-05, Vol.181, p.25-34
• Main Authors: Saha, Mandira, Bandyopadhyay, P.K.
• Format: Article
• Language: English
• Subjects: 18S rDNA; Carassius auratus; Histopathology; Molecular taxonomy; Morphotaxonomy; SEM study; Thelohanellus goldi n. sp
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2018.01.019

[Display omitted] •Description of a new species, Thelohanellus goldi n. sp. on the basis of morphological and morphometrical data along with molecular data.•The severity of infection due to Thelohanellus goldi n. sp. have been assessed by the histopathological changes of fin.•The present paper also deals with the taxonomic description and the prevalence of the myxozoan parasites in goldfish, Carassius auratus in India. A new species of myxozoan, Thelohanellus goldi n. sp. is described using morphological and molecular data, parasitizing the fin filaments from 18 of 25 host specimens (72.5%) of Carassius auratus collected from different ornamental fish farms of India. Mature spore of the new species were oval to spherical in frontal view having rounded posterior ends and tapering anterior end measures 8.7–10.26 (9.50) × 4.10–7.89 (5.84) μm. The single large polar capsule, round to oval in shape but slightly pointed at the anterior end measuring 4.91–7.63 (5.60) × 2.3–3.1 (2.96) μm and located just below the anterior end of the spore. Polar filament only at distal end with 5–6 loose coils. The most differentiating feature from closely related species was carried out by morpho-taxonomic affinities with previously described species which are tremendously supported by molecular taxonomy by partial sequencing of the 18S rDNA gene resulted in a total of 2124 bp fragment of newly obtained small subunit ribosomal RNA gene sequence of the new species which Exhibit 93–95% homogeneity with other closely related species available in GenBank. The BLAST search and high genetic diversity of distance matrix of Myxobolus sp. did not properly match with any available sequences in GenBank and make sister clade with Thelohanellus caudatus and Thelohanellus habibpuri in the Thelohanellus clade including most of Thelohanellus spp. The study of evolutionary history enables us to understand the evolution of modern species and supports some uncertain topologies which are being presented regarding the morphometric analysis. The severity of myxozoan infection has been assessed in this article by observing the histopathological changes of fins of the C. auratus along with the diversity, distribution and taxonomic description of the new Thelohanellus species with their new host and locality records.