Natural killer cell membrane infused biomimetic liposomes for targeted tumor therapy

• Published in: Biomaterials 2018-04, Vol.160, p.124-137
• Main Authors: Pitchaimani, Arunkumar, Nguyen, Tuyen Duong Thanh, Aryal, Santosh
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Biomimetic; Biomimetic Materials; Cell Line; Cell Line, Tumor; Cell membrane; Cell Membrane - chemistry; Cell Survival - drug effects; Doxorubicin; Doxorubicin - administration & dosage; Doxorubicin - chemistry; Drug Carriers - chemistry; Drug Liberation; Female; Fusogenic liposomes; Heterografts; Humans; Killer Cells, Natural - chemistry; Killer Cells, Natural - immunology; Liposomes - chemistry; Mice, Nude; Nanoparticles - chemistry; Natural killer cells; Tissue Distribution; Tumor therapy
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2018.01.018

Therapeutic efficacy of a systemic drug delivery largely depends on the targeting design of the delivery system, which tackles with circulatory traffic and prevents the nonspecific distribution of the drug in the wide range of vital organs. A drawing attention has been given to a biomimetic cloaking of the synthetic drug delivery nanoparticle using mammalian cell-ghosts, which has shown the installment of the biological complexity of the original cells thereby acting as naïve cells, to precisely delivery drug to the intended target. Align towards this direction; we developed a membrane camouflage fusogenic liposomal delivery system “NKsome” for targeted tumor therapy using Natural Killer (NK) cell-ghost, which naturally undergoes immunosurveillance of diseased/stress cells. The engineered NKsome shows successful retention of NK cell membrane-associated targeting protein on its surface. With its excellent biocompatibility, NKsome shows a higher affinity towards cancer than normal cells as demonstrated by in vitro flow-passage assay, and exhibits enhanced tumor homing efficiency in-vivo with an extended plasma residence time of 18 h. Moreover, the therapeutic potential of doxorubicin-loaded NKsome shows promising antitumor activity in vivo against MCF-7 induced tumor model. Overall results illustrate the therapeutic advantages of NK cell biomimicry capable of communicating like immune cells for cooperative drug delivery. [Display omitted] Natural killer cell membrane infused biomimetic liposomes for targeted tumor therapy.