MicroRNA-186 serves as a tumor suppressor in oral squamous cell carcinoma by negatively regulating the protein tyrosine phosphatase SHP2 expression

• Published in: Archives of oral biology 2018-05, Vol.89, p.20-25
• Main Authors: Cai, Zhen, Hao, Xiu-Yan, Liu, Feng-Xin
• Format: Article
• Language: English
• Subjects: Apoptosis; Apoptosis - genetics; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - genetics; Dentistry; Down-Regulation; EGFR; ErbB Receptors - antagonists & inhibitors; ErbB Receptors - metabolism; Female; Genes, Tumor Suppressor; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - metabolism; Humans; Male; MAP Kinase Kinase Kinases - metabolism; MAP Kinase Signaling System; microRNA; MicroRNAs - biosynthesis; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Mouth Neoplasms - genetics; Mouth Neoplasms - metabolism; OSCC; Proliferation; Protein Tyrosine Phosphatase, Non-Receptor Type 11 - biosynthesis; Protein Tyrosine Phosphatase, Non-Receptor Type 11 - genetics; Protein Tyrosine Phosphatase, Non-Receptor Type 11 - metabolism; Proto-Oncogene Proteins c-akt - metabolism; RNA, Messenger - genetics; SHP2; Squamous Cell Carcinoma of Head and Neck; Transfection
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2018.01.016

•MiR-186 expression is significantly downregulated in OSCC tissues and cell lines.•Overexpression of miR-186 induces anti-growth effect in OSCC cells.•Overexpression of miR-186 inhibits EGFR (AKT and ERK) signaling.•MiR-186 directly targets the oncogenic factor, SHP2. MicroRNAs (miRs) have been shown to play critical roles in the pathogenesis of oral squamous cell carcinoma (OSCC), the current study is designed to identify the potential role of miR-186 in OSCC. Realtime polymerase chain reaction was used to determine miR-186 expression in paired tissue samples (OSCC and adjacent normal tissues) and multiple oral cell lines (normal oral keratinocyte HOK cell and OSCC cell lines). Cell viability, colony formation and flow cytometry assays were used to assess the biological function of miR-186. Furthermore, luciferase and western blot assays were used to verify the predicted target of miR-186. We found that miR-186 expression was significantly downregulated in OSCC tissues and cell lines. Overexpression of miR-186 produced an anti-growth effect and induced apoptosis in Tca8113 and SCC-25 cells. Luciferase assay revealed that miR-186 directly targeted PTPN11 (a gene encodes the protein tyrosine phosphatase SHP2) mRNA 3′ untranslated region and suppressed its expression. Consistently, MiR-186 and SHP2 were negatively correlated in OSCC tissues. Consequently, miR-186 inhibited signaling activities of Extracellular Regulated protein Kinases (ERK) and Protein kinase B (AKT), which act downstream of SHP2 and are critical for growth of cancer cells. We identify that miR-186 serves as a tumor suppressor in OSCC. Downregulation of this microRNA may lead to a higher expression of oncogenic factor SHP2, which leads to activation of growth promoting signaling. Thus, miR-186 may be a novel and effective therapeutic agent for the treatment of OSCC.