Molecular characterisation of blaOXA-48 carbapenemase-, extended-spectrum β-lactamase- and Shiga toxin-producing Escherichia coli isolated from farm piglets in India

•First report of blaOXA-48-carrying Escherichia coli in piglets in India.•ESBL-producing E. coli isolates also harboured various antimicrobial resistance genes. The aim of this study was to characterise carbapenemase-, extended-spectrum β-lactamase (ESBL)- and Shiga toxin-producing Escherichia coli...

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Bibliographic Details
Published in:Journal of global antimicrobial resistance. 2018-06, Vol.13, p.201-205
Main Authors: Nirupama, K.R., O.R., Vinodh Kumar, Pruthvishree, B.S., Sinha, D.K., Murugan, M. Senthil, Krishnaswamy, Narayanan, Singh, B.R.
Format: Article
Language:English
Subjects:
Carbapenem
ESBL
Escherichia coli
Faecal sample
Piglet
Shiga toxin
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Summary:•First report of blaOXA-48-carrying Escherichia coli in piglets in India.•ESBL-producing E. coli isolates also harboured various antimicrobial resistance genes. The aim of this study was to characterise carbapenemase-, extended-spectrum β-lactamase (ESBL)- and Shiga toxin-producing Escherichia coli isolated from farm piglets in India. Faecal samples (n=741) from 10 organised pig farms, including non-diarrhoeic (n=546) and diarrhoeic (n=195) piglets, were processed for isolation of carbapenem-resistant and ESBL-producing E. coli. A total of 27 and 243 isolates were phenotypically confirmed as carbapenem-resistant and ESBL-producers, respectively. The meropenem minimum inhibitory concentration (MIC) of carbapenem-resistant isolates ranged from 8–128μg/mL. On genotypic screening of the 27 carbapenem-resistant isolates, 3 isolates were positive for the blaOXA-48 carbapenemase gene; no other carbapenemase genes were detected. The 243 ESBL-producing isolates were positive for blaCTX-M-1 (n=135), qnrA (n=92), qnrB (n=112), qnrS (n=49), tetA (n=42), tetB (n=45) and sul1 (n=43). The Shiga toxin virulence markers stx1 and stx2 were detected in 41 and 38 of the 243 phenotypic ESBL-producing isolates, respectively. Multilocus sequence typing (MLST) of blaOXA-48-positive E. coli isolates showed ST10- and ST5053-like sequence types. This is the first report on the presence of blaOXA-48-carrying E. coli in piglets in India, which pose a potential risk to public health.
ISSN:2213-7165
2213-7173
DOI:10.1016/j.jgar.2018.01.007