Analysis of basidiomycete pigments in situ by Raman spectroscopy

Basidiomycetes, that is, mushroom‐type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlle...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biophotonics 2018-06, Vol.11 (6), p.e201700369-n/a
Main Authors: Tauber, James P., Matthäus, Christian, Lenz, Claudius, Hoffmeister, Dirk, Popp, Jürgen
Format: Article
Language:English
Subjects:
Antioxidants
Basidiocarps
basidiomycetes
Chemical composition
Environmental impact
Fungi
multipartner interactions
natural products
Organic chemistry
Pigmentation
Pigments
Raman spectroscopy
Sample preparation
Secretion
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Basidiomycetes, that is, mushroom‐type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment production is triggered and pigment secretion is generated at the interaction zone. The identification and analysis of these pigments is important to understand the defense mechanism of fungi, which is essential to counteract an uncontrolled spread of harmful species. Usually, a detailed analysis of the pigments is time consuming as it depends on laborious sample preparation and isolation procedures. Furthermore, the applied protocols often influence the chemical integrity of the compound of interest. A possibility to noninvasively investigate the pigmentation is Raman microspectroscopy. The methodology has the potential to analyze the chemical composition of the sample spatially resolved at the interaction zone. After the acquisition of a representative spectroscopic library, the pigment production by basidiomycetes was monitored for during response to different fungi and bacteria. The presented results describe a very efficient noninvasive way of pigment analysis which can be applied with minimal sample preparation. Pigment production by microbes is a common mechanism for defense or interaction with the environment. Identification of these pigments in microbial consortia is complicated as sample preparation is laborious, if possible at all. Raman spectroscopy offers an elegant alternative as compound classes can be reliably identified in high resolution and complex matrices.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201700369