Redox Capacitive Assaying of C‑Reactive Protein at a Peptide Supported Aptamer Interface

Electrochemical immunosensors offer much in the potential translation of a lab based sensing capability to a useful “real world” platform. In previous work we have introduced an impedance-derived electrochemical capacitance spectroscopic analysis as supportive of a reagentless means of reporting on...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2018-03, Vol.90 (5), p.3005-3008
Main Authors: Piccoli, Julia, Hein, Robert, El-Sagheer, Afaf H, Brown, Tom, Cilli, Eduardo M, Bueno, Paulo R, Davis, Jason J
Format: Article
Language:English
Subjects:
Analytical chemistry
Aptamers
C-reactive protein
Capacitance
Chemistry
Electrochemistry
Immunoglobulins
Immunosensors
Interfaces
Peptides
Sensitivity analysis
Sensors
Spectrum analysis
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Summary:Electrochemical immunosensors offer much in the potential translation of a lab based sensing capability to a useful “real world” platform. In previous work we have introduced an impedance-derived electrochemical capacitance spectroscopic analysis as supportive of a reagentless means of reporting on analyte target capture at suitably prepared mixed-component redox-active, antibody-modified interfaces. Herein we directly integrate receptive aptamers into a redox charging peptide support in enabling a label-free low picomolar analytical assay for C-reactive protein with a sensitivity that significantly exceeds that attainable with an analogous antibody interface.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.7b05374