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Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1
Strain KBC1, an anaerobic bacterium, that dechlorinates tetrachloroethene (PCE) to trichloroethene was isolated. This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40 degrees C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this...
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Published in: | Applied microbiology and biotechnology 2006-01, Vol.69 (5), p.543-553 |
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description | Strain KBC1, an anaerobic bacterium, that dechlorinates tetrachloroethene (PCE) to trichloroethene was isolated. This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40 degrees C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this microorganism was identified as a species of the genus Desulfitobacterium. Several species of this genus have been reported to be potent ortho-chlorophenol and PCE dechlorinators; however, the gene coding PCE-specific dehalogenase had not been cloned thus far. In this report, we identified a novel PCE reductive dehalogenase (PrdA) gene from the Desulfitobacterium sp. strain KBC1. These prd genes, including putative membrane anchor protein, were classified as novel type of PCE reductive dehalogenase (approximately 40% homology with the general PCE dehalogenase). It was revealed that the two open reading frames had been transcribed as identical mRNA and were induced strictly in the presence of PCE. This transcriptional regulation appeared to be controlled by the transcriptional activator located downstream of prdAB operon. According to the substrate utility of the strain KBC1 and phylogenetic analysis of PrdA, this microorganism may be expected to play the role of a primary dechlorinator of PCE in the environment. |
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This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40 degrees C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this microorganism was identified as a species of the genus Desulfitobacterium. Several species of this genus have been reported to be potent ortho-chlorophenol and PCE dechlorinators; however, the gene coding PCE-specific dehalogenase had not been cloned thus far. In this report, we identified a novel PCE reductive dehalogenase (PrdA) gene from the Desulfitobacterium sp. strain KBC1. These prd genes, including putative membrane anchor protein, were classified as novel type of PCE reductive dehalogenase (approximately 40% homology with the general PCE dehalogenase). It was revealed that the two open reading frames had been transcribed as identical mRNA and were induced strictly in the presence of PCE. This transcriptional regulation appeared to be controlled by the transcriptional activator located downstream of prdAB operon. According to the substrate utility of the strain KBC1 and phylogenetic analysis of PrdA, this microorganism may be expected to play the role of a primary dechlorinator of PCE in the environment.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-005-0022-x</identifier><identifier>PMID: 16172885</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Amino Acid Motifs ; Amino Acid Sequence ; Bacteria ; Biodegradation, Environmental ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Chlorophenol ; Desulfitobacterium ; Desulfitobacterium - classification ; Desulfitobacterium - enzymology ; Desulfitobacterium - genetics ; Desulfitobacterium - isolation & purification ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes, Regulator - genetics ; Genetics ; Microbiology ; Mission oriented research ; Molecular Sequence Data ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Oxygen - toxicity ; Phylogeny ; Protein Sorting Signals ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Temperature ; Tetrachloroethylene ; Tetrachloroethylene - metabolism ; Transcription, Genetic ; Transcriptional Activation ; Trichloroethylene - metabolism</subject><ispartof>Applied microbiology and biotechnology, 2006-01, Vol.69 (5), p.543-553</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-73e4cb86671e4252773e59d3b55956f8a2eaa22f654c6d859762bcc5c9cee6c93</citedby><cites>FETCH-LOGICAL-c494t-73e4cb86671e4252773e59d3b55956f8a2eaa22f654c6d859762bcc5c9cee6c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/619959527/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/619959527?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11688,27924,27925,36060,36061,44363,74895</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17450564$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16172885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TSUKAGOSHI, Norihiko</creatorcontrib><creatorcontrib>EZAKI, Satoshi</creatorcontrib><creatorcontrib>UENAKA, Tetsuya</creatorcontrib><creatorcontrib>SUZUKI, Nobukazu</creatorcontrib><creatorcontrib>KURANE, Ryuichiro</creatorcontrib><title>Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>Strain KBC1, an anaerobic bacterium, that dechlorinates tetrachloroethene (PCE) to trichloroethene was isolated. This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40 degrees C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this microorganism was identified as a species of the genus Desulfitobacterium. Several species of this genus have been reported to be potent ortho-chlorophenol and PCE dechlorinators; however, the gene coding PCE-specific dehalogenase had not been cloned thus far. In this report, we identified a novel PCE reductive dehalogenase (PrdA) gene from the Desulfitobacterium sp. strain KBC1. These prd genes, including putative membrane anchor protein, were classified as novel type of PCE reductive dehalogenase (approximately 40% homology with the general PCE dehalogenase). It was revealed that the two open reading frames had been transcribed as identical mRNA and were induced strictly in the presence of PCE. This transcriptional regulation appeared to be controlled by the transcriptional activator located downstream of prdAB operon. According to the substrate utility of the strain KBC1 and phylogenetic analysis of PrdA, this microorganism may be expected to play the role of a primary dechlorinator of PCE in the environment.</description><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Bacteria</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Chlorophenol</subject><subject>Desulfitobacterium</subject><subject>Desulfitobacterium - classification</subject><subject>Desulfitobacterium - enzymology</subject><subject>Desulfitobacterium - genetics</subject><subject>Desulfitobacterium - isolation & purification</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. 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Ryuichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>69</volume><issue>5</issue><spage>543</spage><epage>553</epage><pages>543-553</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Strain KBC1, an anaerobic bacterium, that dechlorinates tetrachloroethene (PCE) to trichloroethene was isolated. This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40 degrees C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this microorganism was identified as a species of the genus Desulfitobacterium. Several species of this genus have been reported to be potent ortho-chlorophenol and PCE dechlorinators; however, the gene coding PCE-specific dehalogenase had not been cloned thus far. In this report, we identified a novel PCE reductive dehalogenase (PrdA) gene from the Desulfitobacterium sp. strain KBC1. These prd genes, including putative membrane anchor protein, were classified as novel type of PCE reductive dehalogenase (approximately 40% homology with the general PCE dehalogenase). It was revealed that the two open reading frames had been transcribed as identical mRNA and were induced strictly in the presence of PCE. This transcriptional regulation appeared to be controlled by the transcriptional activator located downstream of prdAB operon. According to the substrate utility of the strain KBC1 and phylogenetic analysis of PrdA, this microorganism may be expected to play the role of a primary dechlorinator of PCE in the environment.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>16172885</pmid><doi>10.1007/s00253-005-0022-x</doi><tpages>11</tpages></addata></record> |
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subjects | Amino Acid Motifs Amino Acid Sequence Bacteria Biodegradation, Environmental Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Chlorophenol Desulfitobacterium Desulfitobacterium - classification Desulfitobacterium - enzymology Desulfitobacterium - genetics Desulfitobacterium - isolation & purification DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes, Regulator - genetics Genetics Microbiology Mission oriented research Molecular Sequence Data Oxidoreductases - genetics Oxidoreductases - metabolism Oxygen - toxicity Phylogeny Protein Sorting Signals RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Temperature Tetrachloroethylene Tetrachloroethylene - metabolism Transcription, Genetic Transcriptional Activation Trichloroethylene - metabolism |
title | Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1 |
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