A new Em-like protein from Lactuca sativa, LsEm1, enhances drought and salt stress tolerance in Escherichia coli and rice

• Published in: Protoplasma 2018-07, Vol.255 (4), p.1089-1106
• Main Authors: Xiang, Dian-Jun, Man, Li-Li, Zhang, Chun-Lan, Peng-Liu, Li, Zhi-Gang, Zheng, Gen-Chang
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Biomedical and Life Sciences; Cell Biology; Chlorophyll; Dehydration; Desiccation; Droughts; E coli; Embryogenesis; Escherichia coli; Escherichia coli - genetics; Flowering; Genes; Genetic engineering; Germination; Homology; L-Lactate dehydrogenase; Lactic acid; Lactuca - genetics; Lactuca - metabolism; LEA protein; Life Sciences; Original Article; Oryza - metabolism; Plant breeding; Plant Proteins - metabolism; Plant Sciences; Proteins; Receptors, Artificial - metabolism; Rice; Salinity tolerance; Salt Tolerance - physiology; Seedlings; Seeds; Signal transduction; Sugar; Superoxide dismutase; Transgenic plants; Zoology
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-018-1207-3

Late embryogenesis abundant (LEA) proteins are closely related to abiotic stress tolerance of plants. In the present study, we identified a novel Em -like gene from lettuce, termed LsEm1 , which could be classified into group 1 LEA proteins, and shared high homology with Cynara cardunculus Em protein. The LsEm1 protein contained three different 20-mer conserved elements (C-element, N-element, and M-element) in the C-termini, N-termini, and middle-region, respectively. The LsEm1 mRNAs were accumulated in all examined tissues during the flowering and mature stages, with a little accumulation in the roots and leaves during the seedling stage. Furthermore, the LsEm1 gene was also expressed in response to salt, dehydration, abscisic acid (ABA), and cold stresses in young seedlings. The LsEm1 protein could effectively reduce damage to the lactate dehydrogenase (LDH) and protect LDH activity under desiccation and salt treatments. The Escherichia coli cells overexpressing the LsEm1 gene showed a growth advantage over the control under drought and salt stresses. Moreover, LsEm1 -overexpressing rice seeds were relatively sensitive to exogenously applied ABA, suggesting that the LsEm1 gene might depend on an ABA signaling pathway in response to environmental stresses. The transgenic rice plants overexpressing the LsEm1 gene showed higher tolerance to drought and salt stresses than did wild-type (WT) plants on the basis of the germination performances, higher survival rates, higher chlorophyll content, more accumulation of soluble sugar, lower relative electrolyte leakage, and higher superoxide dismutase activity under stress conditions. The LsEm1 -overexpressing rice lines also showed less yield loss compared with WT rice under stress conditions. Furthermore, the LsEm1 gene had a positive effect on the expression of the OsCDPK9 , OsCDPK13 , OsCDPK15 , OsCDPK25 , and rab21 ( rab16a ) genes in transgenic rice under drought and salt stress conditions, implying that overexpression of these genes may be involved in the enhanced drought and salt tolerance of transgenic rice. Thus, this work paves the way for improvement in tolerance of crops by genetic engineering breeding.