Anthracene biodegradation by Pseudomonas sp. isolated from a petrochemical sludge landfarming site

• Published in: International biodeterioration & biodegradation 2005-10, Vol.56 (3), p.143-150
• Main Authors: Jacques, Rodrigo J.S., Santos, Eder C., Bento, Fátima M., Peralba, Maria C.R., Selbach, Pedro A., Sá, Enilson L.S., Camargo, Flávio A.O.
• Format: Article
• Language: English
• Subjects: anthracene; biodegradation; bioremediation; Biosurfactant; biosurfactants; industrial wastes; land application; landfarming; petroleum sludge; Phenanthrene; polycyclic aromatic hydrocarbons; Ps. aeruginosa; Ps. citronellolis; Pseudomonas; Pyrene; surfactants; waste disposal
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2005.06.005

Anthracene is a polycyclic aromatic hydrocarbon (PAH) that presents a high pollution potential and health risk and has been used as a model for degradation studies on PAHs because of its relative toxicity. This study aimed to evaluate anthracene degradation by Pseudomonas sp. isolated from a 14-year-old petrochemical sludge landfarming site. Three isolates were selected from 26 by the best growth in anthracene and two of them were identified by 16S rRNA gene sequencing as Ps. aeruginosa and Ps. citronellolis. They showed better growth at pH 7.0 and 30 °C in medium containing up to 2 g anthracene L −1. They were also able to grow in medium containing phenanthrene, pyrene, gasoline and diesel oil. Analysis of anthracene degradation estimated by gas chromatography showed that Ps. aeruginosa isolate 312A had the highest rate of degradation (3.90 mg L −1 day −1), degrading 71% of the anthracene added to the medium (250 mg L −1) after 48 days. Ps. citronellolis 222A showed an intermediate level of degradation (51%), but Ps. aeruginosa 332C degraded only 24.4%. Isolate 312A was also responsible for the highest phenanthrene and pyrene degradation after 48 days. In order to establish the mechanisms involved in the PAH degradation, surfactant production by the isolates was assessed by an emulsification index and reduction of the surface tension in the mineral medium free of cells. Emulsification was not detected, indicating that the isolates did not produce high molecular weight surfactant, although reduction in surface tension indicated production of low molecular weight surfactant compounds. The medium containing Ps. citronellolis 222A showed the highest reduction in surface tension, which could increase anthracene bioavailability for biodegradation. To our knowledge, this is the first report concerning increase of anthracene degradation by surfactants produced by Ps. citronellolis. However, the highest degradation rate shown by Ps. aeruginosa 312A was not related to surfactant production, indicating that some other mechanism could be involved in anthracene degradation. The Pseudomonas isolates may be useful for the study of PAH degradation and for bioremediation purposes.