Detergent assisted ultrasonication aided in situ transesterification for biodiesel production from oleaginous yeast wet biomass

[Display omitted] •The in situ transesterification process was used to convert lipid to biodiesel.•N-Lauroyl sarcosine was used for wet biomass pretreatment.•The ultrasonication was used to enhance FAMEs yield with low reaction time.•There is no impact of N-LS pretreatment and ultrasonication on fat...

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Bibliographic Details
Published in:Bioresource technology 2017-01, Vol.224, p.365-372
Main Authors: Yellapu, Sravan Kumar, Kaur, Rajwinder, Tyagi, Rajeshwar D.
Format: Article
Language:English
Subjects:
Biodiesel
Biofuels
Biomass
Biotechnology - methods
catalysts
detergents
Detergents - pharmacology
Esterification - drug effects
Esters - metabolism
glycerol
In situ transesterification
lipids
Lipids - isolation & purification
methanol
Microbial lipid
N-Lauroyl sarcosine
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - metabolism
Sarcosine - pharmacology
sarcosines
Sulfuric Acids - pharmacology
Time Factors
transesterification
ultrasonic treatment
Ultrasonication
Ultrasonics - methods
viscosity
Water - chemistry
yeasts
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Summary:[Display omitted] •The in situ transesterification process was used to convert lipid to biodiesel.•N-Lauroyl sarcosine was used for wet biomass pretreatment.•The ultrasonication was used to enhance FAMEs yield with low reaction time.•There is no impact of N-LS pretreatment and ultrasonication on fatty acid profile. In situ transesterification of oleaginous yeast wet biomass for fatty acid methyl esters (FAMEs) production using acid catalyst, methanol with or without N-Lauroyl sarcosine (N-LS) treatment was performed. The maximum FAMEs yield obtained with or without N-LS treatment in 24h reaction time was 96.1±1.9 and 71±1.4% w/w, respectively. The N-LS treatment of biomass followed by with or without ultrasonication revealed maximum FAMEs yield of 94.3±1.9% and 82.9±1.8% w/w using methanol to lipid molar ratio 360:1 and catalyst concentration 360mM (64μL H2SO4/g lipid) within 5 and 25min reaction time, respectively. The FAMEs composition obtained in in situ transesterification was similar to that obtained with conventional two step lipid extraction and transesterification process. Biodiesel fuel properties (density, kinematic viscosity, cetane number and total glycerol) were in accordance with international standard (ASTM D6751), which suggests the suitability of biodiesel as a fuel.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.11.037