Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem Cells: Alteration of Gene Expression after In Vitro Cell Culture super( src="corehtml/pmc/pmcents/atyp0255.gif" border="0" alt="D in Box" /> )

• Published in: Molecular biology of the cell 2005-03, Vol.16 (3)
• Main Authors: Boquest, Andrew C, Shahdadfar, Aboulghassem, Froensdal, Katrine, Sigurjonsson, Olafur, Tunheim, Siv H, Collas, Philippe, Brinchmann, Jan E
• Format: Article
• Language: English
• ISSN: 1059-1524; 1059-1524
• DOI: 10.1091/mbc.E04-10-0949

Stromal stem cells proliferate in vitro and may be differentiated along several lineages. Freshly isolated, these cells have been too few or insufficiently pure to be thoroughly characterized. Here, we have isolated two populations of CD45 super(-)CD34 super(+)CD105 super(+) cells from human adipose tissue which could be separated based on expression of CD31. Compared with CD31 super(+) cells, CD31 super(-) cells overexpressed transcripts associated with cell cycle quiescence and stemness, and transcripts involved in the biology of cartilage, bone, fat, muscle, and neural tissues. In contrast, CD31 super(+) cells overexpressed transcripts associated with endothelium and the major histocompatibility complex class II complex. Clones of CD31 super(-) cells could be expanded in vitro and differentiated into cells with characteristics of bone, fat, and neural-like tissue. On culture, transcripts associated with cell cycle quiescence, stemness, certain cytokines and organ specific genes were down- regulated, whereas transcripts associated with signal transduction, cell adhesion, and cytoskeletal components were up-regulated. CD31 super(+) cells did not proliferate in vitro. CD45 super(-)CD34 super(+)CD105 super(+)CD31 super(-) cells from human adipose tissue have stromal stem cell properties which may make them useful for tissue engineering.