Comparative study of host response to chytridiomycosis in a susceptible and a resistant toad species

• Published in: Molecular ecology 2016-11, Vol.25 (22), p.5663-5679
• Main Authors: Poorten, T. J., Rosenblum, E. B.
• Format: Article
• Language: English
• Subjects: Amphibia; amphibian; Anaxyrus boreas; Animal diseases; Animals; Batrachochytrium dendrobatidis; Body weight; Bufo boreas; Bufo marinus; Bufonidae - classification; Bufonidae - genetics; Bufonidae - microbiology; chytridiomycosis; Chytridiomycota - pathogenicity; Comparative studies; comparative transcriptomics; disease biology; Disease Resistance - genetics; emerging diseases; emerging infectious disease; Endangered species; extinction; Fungal infections; fungi; Gene Expression; gene expression regulation; genes; histology; host response; hosts; Infectious diseases; Mycoses - veterinary; Pathogens; Pesticides; population dynamics; Rhinella marina; Skin; Species extinction; Toads; Transcriptome; transcriptomics; Wildlife
• ISSN: 0962-1083; 1365-294X
• DOI: 10.1111/mec.13871

In the past century, recently emerged infectious diseases have become major drivers of species decline and extinction. The fungal disease chytridiomycosis has devastated many amphibian populations and exacerbated the amphibian conservation crisis. Biologists are beginning to understand what host traits contribute to disease susceptibility, but more work is needed to determine why some species succumb to chytridiomycosis while others do not. We conducted an integrative laboratory experiment to examine how two toad species respond to infection with the pathogen Batrachochytrium dendrobatidis in a controlled environment. We selected two toad species thought to differ in susceptibility – Bufo marinus (an invasive and putatively resistant species) and Bufo boreas (an endangered and putatively susceptible species). We measured infection intensity, body weight, histological changes and genomewide gene expression using a custom assay developed from transcriptome sequencing. Our results confirmed that the two species differ in susceptibility with the more susceptible species, B. boreas, showing higher infection intensities, loss in body weight, more dramatic histological changes and larger perturbations in gene expression. We found key differences in skin expression responses in multiple pathways including upregulation of skin integrity‐related genes in the resistant B. marinus. Together, our results show intrinsic differences in host response between related species, which are likely to be important in explaining variation in response to a deadly emerging pathogen in wild populations. Our study also underscores the importance of understanding differences among host species to better predict disease outcomes and reveal generalities about host response to emerging infectious diseases of wildlife.