Assessment of diversity of adenovirus DNA polymerase gene in recreational waters facilitated by ultracentrifugal concentration

Adenoviruses (AdV) are related to respiratory and gastrointestinal diseases in animals and human beings. Their wide genetic diversity in water bodies and their resistance to environmental conditions allow the use of AdV as a reliable marker for detection of fecal contamination. In this work, the div...

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Bibliographic Details
Published in:Journal of water and health 2018-02, Vol.16 (1), p.102-111
Main Authors: Girardi, Viviane, Demoliner, Meriane, Rigotto, Caroline, Schneider, Vania Elisabete, Paesi, Suelen, Spilki, Fernando Rosado
Format: Article
Language:English
Subjects:
Adenoviruses
Animal diseases
Contamination
Creeks & streams
Deoxyribonucleic acid
Detection
DNA
DNA polymerase
DNA-directed DNA polymerase
Effluents
Environmental conditions
Fecal coliforms
Gastrointestinal diseases
Genetic diversity
Genetic variation
Identification
Infections
Nucleotide sequence
Recreation
Recreational waters
Rivers
Sanitation
Species
Viruses
Water resistance
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Summary:Adenoviruses (AdV) are related to respiratory and gastrointestinal diseases in animals and human beings. Their wide genetic diversity in water bodies and their resistance to environmental conditions allow the use of AdV as a reliable marker for detection of fecal contamination. In this work, the diversity of AdV along Belo Stream - in the city of Caxias do Sul, Rio Grande do Sul, Brazil - was evaluated. Samples were compared in both concentrated and unconcentrated forms. The identification of different AdV species was performed by amplifying a partial sequence of the DNA polymerase gene. AdV was detected in 24 out of 55 concentrated samples (43.6%) and the following species were identified: human adenovirus (HAdV) species C (4/55; 7.2%), D (6/55; 10.9%), E (2/55; 3.6%), and F (9/55; 16.3%). AdV related to other mammalian hosts, such as bovine adenovirus (1/55, 1.8%) and murine adenovirus (2/55, 3.6%), have also been identified; 23.6% (13/55) of the unconcentrated samples were positive, and identified as HAdV species C (6/55, 10.9%), D (1/55, 1.8%), and F (6/55, 10.9%). Results obtained evidenced the presence and the great diversity of AdV, mainly of human origin, circulating in Belo Stream. As expected, the concentration step performed helped to detect AdV in more samples.
ISSN:1477-8920
1996-7829
DOI:10.2166/wh.2017.144