Validating a probe from GhSERK1 gene for selection of cotton genotypes with somatic embryogenic capacity

•A probe of GhSERK1 gene as marker for identification of embryogenic cotton plants.•Identification of embryogenic plants from zygote tissue.•Availability of specific primers designed from conserved regions of SERK genes. Substantial progress is being reported in the techniques for plant transformati...

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Bibliographic Details
Published in:Journal of biotechnology 2018-03, Vol.270, p.44-50
Main Authors: da Cunha Soares, Taiza, da Silva, Carliane Rebeca Coelho, Chagas Carvalho, Julita Maria Frota, Cavalcanti, José Jaime Vasconcelos, de Lima, Liziane Maria, de Albuquerque Melo Filho, Péricles, Severino, Liv Soares, dos Santos, Roseane Cavalcanti
Format: Article
Language:English
Subjects:
Blotting
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genotype
Gossypium - embryology
Gossypium - genetics
Gossypium - metabolism
Gossypium hirsutum L
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Somatic Embryogenesis Techniques - methods
Plants, Genetically Modified - growth & development
Plants, Genetically Modified - metabolism
Protein Kinases - genetics
Protein Kinases - metabolism
Seeds - embryology
Seeds - genetics
Selection, Genetic
Somatic embryogenesis
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Summary:•A probe of GhSERK1 gene as marker for identification of embryogenic cotton plants.•Identification of embryogenic plants from zygote tissue.•Availability of specific primers designed from conserved regions of SERK genes. Substantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures. High expression of transcripts was found in embryogenic genotypes, and the results were confirmed by the RT-PCR-blotting using a non-radioactive probe. The regeneration ability was confirmed in embryogenic genotypes. We confirmed that GhSERK1 can be used as marker for estimating the somatic embryogenesis ability of cotton plants.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2018.02.002