Sex steroid receptors profiling is influenced by nandrolone decanoate in the ampulla of the fallopian tube: Post-treatment and post-recovery analyses

• Published in: Tissue & cell 2018-02, Vol.50, p.79-88
• Main Authors: Andrade, G.H.B., Simão, V.A., Souza, B.R., Chuffa, L.G.A., Camargo, I.C.C.
• Format: Article
• Language: English
• Subjects: Cytoplasm; Epithelial cells; Estrus cycle; Fallopian tube; Immunohistochemistry; Medical treatment; Mineral oils; Muscles; Nandrolone decanoate; Nortestosterone; Nuclei; Nuclei (cytology); Rats; Receptors; Recovery; Sex steroids receptors; Steroid hormone receptors; Steroid hormones; Steroids; Stroma; Testosterone
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2018.01.001

•Rats received different doses of Nandrolone Decanoate.•The ampulla of fallopian tube was evaluated after treatment and recovery periods.•Sex steroids receptors are differentially expressed following different doses of ND.•The ampulla presented histomorphometric and immunohistochemical changes in a dose-independent manner after treatment and recovery periods. Anabolic androgenic steroids (AAS) are recommended for therapeutic clinic, but their use has increased in recent decades for aesthetic reasons. No study has evaluated the impact of AAS in the fallopian tube, after treatment and recovery periods. Herein, the aim of study was to investigate the effects of Nandrolone Decanoate (ND), administered in different doses (1.87; 3.75; 7.5 and 15 mg/kg) on the ampulla of the fallopian tube in rats, following post-treatment (PT; 15 consecutive days) and post-recovery (PR; 30 consecutive days) periods. The control group received mineral oil. Estrous cycle was monitored daily during both periods and in sequence the rats (n = 8/group/period) were killed. All ND-treated animals showed estral acyclicity during the PT and PR periods, but the histomorphometric changes in the fallopian tube varied according to the ND dose level. The expression of AR, ERα and ERβ varied in the nucleus and cytoplasm of epithelial cells. No AR expression was observed in the stroma. The muscle cells exhibited variation in immunostaining. In conclusion, ND promoted histomorphometric and immunohistochemical changes in the ampullary portion of the fallopian tube after treatment and recovery periods in a dose-independent manner.