FT‐Raman spectroscopy, µ‐EDXRF spectrometry, and microhardness analysis of the dentin of primary and permanent teeth

The chemical compositions (organic and inorganic contents) and mechanical behaviors of the dentin of permanent and deciduous teeth were analyzed and compared using X‐ray fluorescence spectrometry (µ‐EDXRF) Fourier transform Raman spectroscopy (FT‐Raman) and a microhardness test (HD). Healthy fresh h...

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Bibliographic Details
Published in:Microscopy research and technique 2018-05, Vol.81 (5), p.509-514
Main Authors: Torres, Carolina Paes, Miranda Gomes‐Silva, Jaciara, Menezes‐Oliveira, Maria Angélica Hueb, Silva Soares, Luís Eduardo, Palma‐Dibb, Regina Guenka, Borsatto, Maria Cristina, Yang, Mingying
Format: Article
Language:English
Subjects:
Analysis of Variance
Dental Enamel - chemistry
Dentin
Dentin - chemistry
dentine
EDX
Fluorescence
Fourier transforms
FTIR
Humans
Mechanical properties
Microhardness
Microscopy, Electron, Scanning
Molars
Physical properties
Raman spectroscopy
Scientific imaging
Spectrometry
Spectrometry, X-Ray Emission
Spectroscopy
Spectrum Analysis, Raman - methods
Substrates
Teeth
Tooth, Deciduous - chemistry
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Summary:The chemical compositions (organic and inorganic contents) and mechanical behaviors of the dentin of permanent and deciduous teeth were analyzed and compared using X‐ray fluorescence spectrometry (µ‐EDXRF) Fourier transform Raman spectroscopy (FT‐Raman) and a microhardness test (HD). Healthy fresh human primary and permanent molars (n = 10) were selected, The buccal surfaces facing upwards were stabilized in an acrylic plate, flattened, polished, and submitted to the µ‐EDXRF, FT‐Raman, and HD analysis. The results of the analysis were subjected to ANOVAs and Mann‐Whitney U/Student's t multiple comparisons tests. The data showed similar values for the dentin of the primary and permanent teeth in P content, organic content (amide I peak), inorganic content ( PO43– – 430 and 590), and microhardness, Nevertheless, Ca content and Ca/P weight ratio were higher, and the CO32– peak was lower in the dentin of the permanent teeth compared to primary teeth. It be concluded that despite permanent teeth showed more Ca element, both substrates showed similar behavior of chemical and physical properties. The dentine substrate of primary teeth presents the lowest Ca content and Ca/P weight ratio and the permanent human dentine showed the lowest CO32– peak.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.23005