Loading…

Gene Expression Modulation in A549 Human Lung Cells in Response to Combustion-Generated Nano-Sized Particles

:  High levels of ambient air pollution are associated in humans with aggravation of asthma and of respiratory and cardiopulmonary morbidity; long‐term exposures to particulate matter (PM) have been linked to possible increases in lung cancer risk, chronic respiratory disease, and increased death ra...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2006-12, Vol.1091 (1), p.170-183
Main Authors: ARENZ, ANDREA, HELLWEG, CHRISTINE E., STOJICIC, NEVENA, BAUMSTARK-KHAN, CHRISTA, GROTHEER, HORST-HENNING
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary::  High levels of ambient air pollution are associated in humans with aggravation of asthma and of respiratory and cardiopulmonary morbidity; long‐term exposures to particulate matter (PM) have been linked to possible increases in lung cancer risk, chronic respiratory disease, and increased death rates. The Biodiagnostics Group of the DLR Institute of Aerospace Medicine develops cellular test systems capable of monitoring the biological consequences of environmental conditions on humans already on cellular and molecular level. Such bioassays rely on the receptor–reporter principle, where cell lines are transfected with plasmids carrying a reporter gene under control of environment‐dependent promoters (receptor), which play a key role in regulating gene expressions in response to extracellular signals. We developed the recombinant human lung epithelial cell line A549‐NF‐κB‐EGFP/Neo carrying a genetically encoded fluorescent indicator for monitoring activation of the NF‐κB signaling pathway in living cells in response to genotoxic and cytotoxic environmental influences. With this cell line we screened several candidate human radiation‐responsive genes (GADD45β, CDKN1A) and NF‐κB‐dependent genes (IL‐6, NFκBIA, and pNF‐κB‐EGFP) for gene expression changes by quantitative reverse transcriptase polymerase chain reaction (qRT‐PCR) assay, using cDNA obtained from total RNA isolated at various time points after exposure to combustion generated nano‐sized particle samples.
ISSN:0077-8923
1749-6632
1930-6547
DOI:10.1196/annals.1378.064