Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus

Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 × 10 2 mouse unit (MU) per gram...

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Bibliographic Details
Published in:Toxicon (Oxford) 2008-07, Vol.52 (1), p.55-61
Main Authors: Wang, Xiao-Jie, Yu, Ren-Cheng, Luo, Xuan, Zhou, Ming-Jiang, Lin, Xiang-Tian
Format: Article
Language:English
Subjects:
16S rDNA
Aeromonas
Animal poisons toxicology. Antivenoms
Animals
Bacteria
Bacteria - isolation & purification
Bacteria - pathogenicity
Biological and medical sciences
Chromatography, High Pressure Liquid
ELISA
Gastropoda
Gastropoda - microbiology
Gastropoda - pathogenicity
LC-MS
Marine
Marinomonas
Mass Spectrometry
Medical sciences
Mice
Nassarius
Nassarius semiplicatus
Shewanella
Tenacibaculum
Tetrodotoxin
Tetrodotoxin - analysis
Tetrodotoxin - biosynthesis
Tetrodotoxin - toxicity
Toxicology
Vibrio
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Summary:Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 × 10 2 mouse unit (MU) per gram of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2008.04.170