Loading…
Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus
Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 × 10 2 mouse unit (MU) per gram...
Saved in:
| Published in: | Toxicon (Oxford) 2008-07, Vol.52 (1), p.55-61 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3 |
| container_end_page | 61 |
| container_issue | 1 |
| container_start_page | 55 |
| container_title | Toxicon (Oxford) |
| container_volume | 52 |
| creator | Wang, Xiao-Jie Yu, Ren-Cheng Luo, Xuan Zhou, Ming-Jiang Lin, Xiang-Tian |
| description | Bacteria isolated from a highly toxic sample of gastropod
Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2
×
10
2
mouse unit (MU) per gram of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus
Vibrio, and the others were related to genus
Shewanella,
Marinomonas,
Tenacibaculum and
Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in
N. semiplicatus. |
| doi_str_mv | 10.1016/j.toxicon.2008.04.170 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20025668</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0041010108003590</els_id><sourcerecordid>20025668</sourcerecordid><originalsourceid>FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3</originalsourceid><addsrcrecordid>eNqFkE1v1DAQhi0EokvhJ4B8gVuCnS8nJ4QqKEgVXMrZcsbjrVeJvfUkVfvv8bIRHDlZsp_39czD2FspSilk9_FQLvHRQwxlJURfiqaUSjxjO9mroahlK56znRCNLETGL9grooMQou6H7iW7kH2r6ko1O3Z_m1tCQZAQgw97boLl3mJYvPNgFh8Dj46PBhZM3nBPcTILWu5SnPmd399NT_zPJHw2yQfke0NLisdo-Q9DlO9W4oSzP06nvpVesxfOTIRvtvOS_fr65fbqW3Hz8_r71eebAppBLIWzdhz7anAwAri6tqOzSja2a0WnTK9GBXUDbuyVtYMBHIdukM51aqikhPx4yT6ce48p3q9Ii549AU6TCRhX0llb1XZdn8H2DEKKRAmdPiafl3nSUuiTa33Qm-tTqNei0dl1zr3bPljHGe2_1CY3A-83wBCYySUTwNNfrhKtqob2xH06c5h1PHhMmsBjALQ-ISzaRv-fUX4DMyqkKA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20025668</pqid></control><display><type>article</type><title>Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus</title><source>ScienceDirect Journals</source><creator>Wang, Xiao-Jie ; Yu, Ren-Cheng ; Luo, Xuan ; Zhou, Ming-Jiang ; Lin, Xiang-Tian</creator><creatorcontrib>Wang, Xiao-Jie ; Yu, Ren-Cheng ; Luo, Xuan ; Zhou, Ming-Jiang ; Lin, Xiang-Tian</creatorcontrib><description>Bacteria isolated from a highly toxic sample of gastropod
Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2
×
10
2
mouse unit (MU) per gram of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus
Vibrio, and the others were related to genus
Shewanella,
Marinomonas,
Tenacibaculum and
Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in
N. semiplicatus.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/j.toxicon.2008.04.170</identifier><identifier>PMID: 18573274</identifier><identifier>CODEN: TOXIA6</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>16S rDNA ; Aeromonas ; Animal poisons toxicology. Antivenoms ; Animals ; Bacteria ; Bacteria - isolation & purification ; Bacteria - pathogenicity ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; ELISA ; Gastropoda ; Gastropoda - microbiology ; Gastropoda - pathogenicity ; LC-MS ; Marine ; Marinomonas ; Mass Spectrometry ; Medical sciences ; Mice ; Nassarius ; Nassarius semiplicatus ; Shewanella ; Tenacibaculum ; Tetrodotoxin ; Tetrodotoxin - analysis ; Tetrodotoxin - biosynthesis ; Tetrodotoxin - toxicity ; Toxicology ; Vibrio</subject><ispartof>Toxicon (Oxford), 2008-07, Vol.52 (1), p.55-61</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3</citedby><cites>FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20572954$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18573274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xiao-Jie</creatorcontrib><creatorcontrib>Yu, Ren-Cheng</creatorcontrib><creatorcontrib>Luo, Xuan</creatorcontrib><creatorcontrib>Zhou, Ming-Jiang</creatorcontrib><creatorcontrib>Lin, Xiang-Tian</creatorcontrib><title>Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>Bacteria isolated from a highly toxic sample of gastropod
Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2
×
10
2
mouse unit (MU) per gram of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus
Vibrio, and the others were related to genus
Shewanella,
Marinomonas,
Tenacibaculum and
Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in
N. semiplicatus.</description><subject>16S rDNA</subject><subject>Aeromonas</subject><subject>Animal poisons toxicology. Antivenoms</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - pathogenicity</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>ELISA</subject><subject>Gastropoda</subject><subject>Gastropoda - microbiology</subject><subject>Gastropoda - pathogenicity</subject><subject>LC-MS</subject><subject>Marine</subject><subject>Marinomonas</subject><subject>Mass Spectrometry</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Nassarius</subject><subject>Nassarius semiplicatus</subject><subject>Shewanella</subject><subject>Tenacibaculum</subject><subject>Tetrodotoxin</subject><subject>Tetrodotoxin - analysis</subject><subject>Tetrodotoxin - biosynthesis</subject><subject>Tetrodotoxin - toxicity</subject><subject>Toxicology</subject><subject>Vibrio</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkE1v1DAQhi0EokvhJ4B8gVuCnS8nJ4QqKEgVXMrZcsbjrVeJvfUkVfvv8bIRHDlZsp_39czD2FspSilk9_FQLvHRQwxlJURfiqaUSjxjO9mroahlK56znRCNLETGL9grooMQou6H7iW7kH2r6ko1O3Z_m1tCQZAQgw97boLl3mJYvPNgFh8Dj46PBhZM3nBPcTILWu5SnPmd399NT_zPJHw2yQfke0NLisdo-Q9DlO9W4oSzP06nvpVesxfOTIRvtvOS_fr65fbqW3Hz8_r71eebAppBLIWzdhz7anAwAri6tqOzSja2a0WnTK9GBXUDbuyVtYMBHIdukM51aqikhPx4yT6ce48p3q9Ii549AU6TCRhX0llb1XZdn8H2DEKKRAmdPiafl3nSUuiTa33Qm-tTqNei0dl1zr3bPljHGe2_1CY3A-83wBCYySUTwNNfrhKtqob2xH06c5h1PHhMmsBjALQ-ISzaRv-fUX4DMyqkKA</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Wang, Xiao-Jie</creator><creator>Yu, Ren-Cheng</creator><creator>Luo, Xuan</creator><creator>Zhou, Ming-Jiang</creator><creator>Lin, Xiang-Tian</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20080701</creationdate><title>Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus</title><author>Wang, Xiao-Jie ; Yu, Ren-Cheng ; Luo, Xuan ; Zhou, Ming-Jiang ; Lin, Xiang-Tian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>16S rDNA</topic><topic>Aeromonas</topic><topic>Animal poisons toxicology. Antivenoms</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - pathogenicity</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>ELISA</topic><topic>Gastropoda</topic><topic>Gastropoda - microbiology</topic><topic>Gastropoda - pathogenicity</topic><topic>LC-MS</topic><topic>Marine</topic><topic>Marinomonas</topic><topic>Mass Spectrometry</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Nassarius</topic><topic>Nassarius semiplicatus</topic><topic>Shewanella</topic><topic>Tenacibaculum</topic><topic>Tetrodotoxin</topic><topic>Tetrodotoxin - analysis</topic><topic>Tetrodotoxin - biosynthesis</topic><topic>Tetrodotoxin - toxicity</topic><topic>Toxicology</topic><topic>Vibrio</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiao-Jie</creatorcontrib><creatorcontrib>Yu, Ren-Cheng</creatorcontrib><creatorcontrib>Luo, Xuan</creatorcontrib><creatorcontrib>Zhou, Ming-Jiang</creatorcontrib><creatorcontrib>Lin, Xiang-Tian</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiao-Jie</au><au>Yu, Ren-Cheng</au><au>Luo, Xuan</au><au>Zhou, Ming-Jiang</au><au>Lin, Xiang-Tian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>52</volume><issue>1</issue><spage>55</spage><epage>61</epage><pages>55-61</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>Bacteria isolated from a highly toxic sample of gastropod
Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2
×
10
2
mouse unit (MU) per gram of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus
Vibrio, and the others were related to genus
Shewanella,
Marinomonas,
Tenacibaculum and
Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in
N. semiplicatus.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18573274</pmid><doi>10.1016/j.toxicon.2008.04.170</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-0101 |
| ispartof | Toxicon (Oxford), 2008-07, Vol.52 (1), p.55-61 |
| issn | 0041-0101 1879-3150 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20025668 |
| source | ScienceDirect Journals |
| subjects | 16S rDNA Aeromonas Animal poisons toxicology. Antivenoms Animals Bacteria Bacteria - isolation & purification Bacteria - pathogenicity Biological and medical sciences Chromatography, High Pressure Liquid ELISA Gastropoda Gastropoda - microbiology Gastropoda - pathogenicity LC-MS Marine Marinomonas Mass Spectrometry Medical sciences Mice Nassarius Nassarius semiplicatus Shewanella Tenacibaculum Tetrodotoxin Tetrodotoxin - analysis Tetrodotoxin - biosynthesis Tetrodotoxin - toxicity Toxicology Vibrio |
| title | Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T17%3A41%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Toxin-screening%20and%20identification%20of%20bacteria%20isolated%20from%20highly%20toxic%20marine%20gastropod%20Nassarius%20semiplicatus&rft.jtitle=Toxicon%20(Oxford)&rft.au=Wang,%20Xiao-Jie&rft.date=2008-07-01&rft.volume=52&rft.issue=1&rft.spage=55&rft.epage=61&rft.pages=55-61&rft.issn=0041-0101&rft.eissn=1879-3150&rft.coden=TOXIA6&rft_id=info:doi/10.1016/j.toxicon.2008.04.170&rft_dat=%3Cproquest_cross%3E20025668%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c490t-fddbb829fcbccf33dbfd714d65067a87b7c34cfb87dd9aceb9691ff679211c7c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20025668&rft_id=info:pmid/18573274&rfr_iscdi=true |