PI3K/AKT signaling pathway involvement in fluoride-induced apoptosis in C2C12 cells

To investigate the mechanisms of fluoride-induced apoptosis, a fluoride-induced C2C12 skeletal muscle cell (C2C12 cell) model was established in this study, and the viability of the C2C12 cells was measured using an MTT assay. Cell morphological changes were observed via haematoxylin and eosin stain...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2018-05, Vol.199, p.297-302
Main Authors: Zhou, Bian-hua, Tan, Pan-pan, Jia, Liu-shu, Zhao, Wen-peng, Wang, Ji-cang, Wang, Hong-wei
Format: Article
Language:English
Subjects:
AKT
Apoptosis
C2C12 cells
Fluoride
PI3K
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Summary:To investigate the mechanisms of fluoride-induced apoptosis, a fluoride-induced C2C12 skeletal muscle cell (C2C12 cell) model was established in this study, and the viability of the C2C12 cells was measured using an MTT assay. Cell morphological changes were observed via haematoxylin and eosin staining and transmission electron microscopy. Apoptosis was monitored through Hoechst staining. The mRNA and protein expression of PI3K, PDK1, AKT1, BAD, Bcl-2, Bax and caspase-9 were detected through real-time PCR and western blotting, respectively. The results showed that the survival rates of C2C12 cells decreased gradually with an increasing fluoride doses. The C2C12 cell structure was seriously damaged by fluoride, presenting with pyknosis, mitochondrial ridge disruption and swollen endoplasmic reticulum. Furthermore, the expression of mRNA in PI3K, BAD, Bcl-2, Bax and caspase-9 were significantly increased in the fluoride group (P 
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2018.02.057