In vitro fermentation of xylo-oligosaccharides from corn cobs autohydrolysis by Bifidobacterium and Lactobacillus strains

The selective release of soluble oligomeric structures from corn cobs (CC) by autohydrolysis was optimized using the severity factor (log R 0), and a maximum xylo-oligosaccharides (XOS) production was obtained at log R 0=3.75. Gel filtration chromatography was employed to separate oligosaccharides (...

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Bibliographic Details
Published in:Food science & technology 2007-08, Vol.40 (6), p.963-972
Main Authors: Moura, Patrícia, Barata, Rosário, Carvalheiro, Florbela, Gírio, Francisco, Loureiro-Dias, Maria C., Esteves, M. Paula
Format: Article
Language:English
Subjects:
Autohydrolysis
automation
Bifidobacterium adolescentis
Bifidobacterium longum
Biological and medical sciences
Cereal and baking product industries
Corn cobs
dietary fiber
fermentation
Food industries
Food microbiology
food processing wastes
Fundamental and applied biological sciences. Psychology
hydrolysis
in vitro culture
lactic acid bacteria
Lactobacillus
Lactobacillus brevis
Lactobacillus fermentum
Prebiotic
prebiotics
starter cultures
waste utilization
Xylo-oligosaccharide
xylobiose
xylooligosaccharides
xylotertraose
xyltriose
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Summary:The selective release of soluble oligomeric structures from corn cobs (CC) by autohydrolysis was optimized using the severity factor (log R 0), and a maximum xylo-oligosaccharides (XOS) production was obtained at log R 0=3.75. Gel filtration chromatography was employed to separate oligosaccharides (OS) from the crude hydrolysate and two fractions with average degrees of polymerization (DP) of 3–4 and 5–6 were compared with commercial XOS in their ability to promote the growth of Bifidobacterium adolescentis, B. longum, Lactobacillus brevis and L. fermentum as carbon and energy sources. Among the tested strains, B. adolescentis and L. brevis displayed the highest growth and XOS consumption, in contrast to B. longum and L. fermentum whose overall growth on XOS was low. XOS mixtures from CC autohydrolysis, mainly constituted by xylotriose and xylotetraose, were fermented by B. adolescentis as well as commercial XOS containing essentially xylobiose, whereas L. brevis preferred XOS with an average DP of 2. However, an increase in XOS chain length to DP 5–6 clearly reduced the extension to which B. adolescentis utilized these OS. This study showed that XOS of low molecular weight from CC autohydrolysis exhibit a potential bifidogenic capability similar to commercial XOS.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2006.07.013