Alga-lytic activity of Pseudomonas fluorescens against the red tide causing marine alga Heterosigma akashiwo (Raphidophyceae)

A bacterial strain, HAK-13, exhibited strongest activity against Heterosigma akashiwo and was capable of controlling this bloom forming phytoplankton. Based on 16S rDNA sequences and biochemical and morphological characteristics, the strain HAK-13 was determined to be Pseudomonas fluorescens on the...

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Bibliographic Details
Published in:Biological control 2007-06, Vol.41 (3), p.296-303
Main Authors: Kim, Jeong-Dong, Kim, Bora, Lee, Choul-Gyun
Format: Article
Language:English
Subjects:
Akashiwo
Alexandrium tamarense
Alga-lytic activity
alga-lytic bacteria
Algae
algae control
algal blooms
biological control agents
Cochlodinium polykrikoides
cytolysis
growth and development
growth inhibition
Gymnodinium catenatum
Harmful algal blooms (HABs)
Heterosigma akashiwo
host range
phytoplankton
Prorocentrum dentatum
Pseudomonas fluorescens
Raphidophyceae
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Summary:A bacterial strain, HAK-13, exhibited strongest activity against Heterosigma akashiwo and was capable of controlling this bloom forming phytoplankton. Based on 16S rDNA sequences and biochemical and morphological characteristics, the strain HAK-13 was determined to be Pseudomonas fluorescens on the basis of 99.9% similarity with reference strains in the DNA databases. The growth of H. akashiwo was strongly suppressed by HAK-13 in all growth phases, with the strongest alga-lytic activity noted against harmful bloom-forming species in the exponential stage (6–22 days). Host range tests showed that HAK-13 also significantly inhibited the growth of Alexandrium tamarense and Cochlodinium polykrikoides but could not destroy Gymnodinium catenatum. P. fluorescens HAK-13 indirectly attacked H. akashiwo by alga-lytic substances that might be located at the compartment of cytoplasmic membrane of the bacterium at a level of 45.86 units/mg of specific activity. The results indicated that P. fluorescens HAK-13 caused cell lysis and death of H. akashiwo, A. tamarense, and C. polykrikoides dramatically and Prorocentrum dentatum slightly. Therefore, P. fluorescens HAK-13 has potential for use as a selective biocontrol of harmful algal blooms.
ISSN:1049-9644
1090-2112
DOI:10.1016/j.biocontrol.2007.02.010