Toxoplasma gondii: Effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro

The major aim to the present study was to determine the effects of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom, on invasion and replication of Toxoplasma gondii in human fibroblasts in vitro. Neuwiedase treatment was done on host cells previously infected with T. gond...

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Published in:Experimental parasitology 2008-12, Vol.120 (4), p.391-396
Main Authors: Bastos, Luciana Machado, Júnior, Robson J. Oliveira, Silva, Deise Aparecida Oliveira, Mineo, José Roberto, Vieira, Carlos Ueira, Teixeira, David Nascimento Silva, Homsi-Brandeburgo, Maria Inês, Rodrigues, Veridiana Melo, Hamaguchi, Amélia
Format: Article
Language:English
Subjects:
Amphibia. Reptilia
Animals
Biological and medical sciences
Bothrops neuwiedi
Cell Survival - drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - immunology
Fibroblasts - parasitology
Fundamental and applied biological sciences. Psychology
Human fibroblasts
Humans
Inhibitory Concentration 50
Interleukin-8 - biosynthesis
Interleukin-8 - drug effects
Life cycle. Host-agent relationship. Pathogenesis
Male
Metalloendopeptidases - toxicity
Mice
Neuwiedase
Protozoa
Snake venom
Toxoplasma - drug effects
Toxoplasma - physiology
Toxoplasma gondii
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
Viper Venoms - toxicity
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Summary:The major aim to the present study was to determine the effects of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom, on invasion and replication of Toxoplasma gondii in human fibroblasts in vitro. Neuwiedase treatment was done on host cells previously infected with T. gondii or on parasite before fibroblast infection. When treatments were done after or before infection, infection rates were inhibited in 71% and 61%, respectively. Considering that therapy protocols currently used in T. gondii infection cause considerable side effects, particularly in immunocompromised individuals and pregnant women, the results of neuwiedase treatment described herein could be taken into account for the development of new synthetic therapeutic agents, mainly due to the capacity of this enzyme to degrade extracellular matrix components, such as laminin, fibronectin and type I collagen, which is important to interfere in T. gondii host cell invasion.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2008.09.008