Cell-Based Electrochemical Assay for Endotoxin Using a Secreted Alkaline Phosphatase Reporter System

We developed a cell-based assay device for the detection of endotoxin, the potentially toxic compound that induces septic shock. Genetically-engineered cells that secrete alkaline phosphatase (SEAP) on exposure to endotoxin were cultured in an electrochemical cell device in medium containing p-amino...

Full description

Saved in:
Bibliographic Details
Published in:Denki kagaku oyobi kōgyō butsuri kagaku 2008/08/05, Vol.76(8), pp.525-528
Main Authors: INOUE, Kumi Y., YASUKAWA, Tomoyuki, SHIKU, Hitoshi, MATSUE, Tomokazu
Format: Article
Language:English
Subjects:
Amperometry
Cell-based Sensor
Lipopolysaccharide
Secreted Alkaline Phosphatase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We developed a cell-based assay device for the detection of endotoxin, the potentially toxic compound that induces septic shock. Genetically-engineered cells that secrete alkaline phosphatase (SEAP) on exposure to endotoxin were cultured in an electrochemical cell device in medium containing p-aminophenyl phosphate and various concentrations of endotoxin. After 24 hr incubation, p-aminophenol (pAP), generated by SEAP-catalyzed hydrolysis, was detected by amperometry at +0.35 V. The amperometric response increased with the concentration of endotoxin in the range of 0.01–1 ng/ml.
ISSN:1344-3542
2186-2451
DOI:10.5796/electrochemistry.76.525