Rapid adaptation of phytoplankters to geothermal waters is achieved by single mutations: were extreme environments 'Noah's Arks' for photosynthesizers during the Neoproterozoic 'snowball Earth'?

• Published in: The New phytologist 2008-12, Vol.180 (4), p.922-932
• Main Authors: Costas, Eduardo, Flores-Moya, Antonio, López-Rodas, Victoria
• Format: Article
• Language: English
• Subjects: adaptation; Adaptation, Biological; Algae; Cell physiology; Chlorophyta - genetics; Chlorophyta - physiology; Cultured cells; Cyanobacteria; Cyanophyta; Dictyosphaerium; Dictyosphaerium chlorelloides; Ecosystem; Evolution, Molecular; Fresh Water - microbiology; Genetic mutation; geothermal waters; Hot springs; Hot Springs - microbiology; Ice Cover; Microalgae; Microcystis; Microcystis - genetics; Microcystis - physiology; Microcystis aeruginosa; Mutation; Neoproterozoic 'snowball Earth'; Phytoplankton - genetics; Phytoplankton - physiology; Ponds; Selection, Genetic; Snowball Earth hypothesis; Stress, Physiological; survival photosynthesizers; Water samples
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/j.1469-8137.2008.02620.x

Geothermal waters often support remarkable communities of microalgae and cyanobacteria apparently living at the extreme limits of their tolerance. Little is known about the mechanisms allowing adaptation of mesophilic phytoplankters to such extreme conditions, but recent studies are challenging many preconceived notions about this. The aim of this study was to analyse mechanisms allowing adaptation of mesophilic microalgae and cyanobacteria to stressful geothermal waters. To distinguish between the pre-selective or post-selective origin of adaptation processes allowing the proliferation of mesophilic phytoplankters in geothermal waters, several Luria-Delbrück fluctuation analysis were performed with the microalga Dictyosphaerium chlorelloides and the cyanobacterium Microcystis aeruginosa, both isolated from nonextreme waters. Geothermal waters from seven places in Italy and five icebound places at Los Andes in Argentina were used as selective agents. Physiological adaptation was achieved in the least toxic waters. In contrast, rapid genetic adaptation was observed in waters ostensibly lethal for the experimental organisms. This adaptation was achieved as consequence of single mutations at one locus. It was hypothesized that a similar mechanism of rapid genetic adaptation could explain the survival of photosynthetic life during the Neoproterozoic 'snowball Earth,' where geothermal refuges such as those studied could have been 'Noah's Arks' for microalgae and cyanobacteria.