Uptake and depuration of the anti-depressant fluoxetine by the Japanese medaka ( Oryzias latipes)

• Published in: Chemosphere (Oxford) 2008-12, Vol.74 (1), p.125-130
• Main Authors: Paterson, Gordon, Metcalfe, Chris D.
• Format: Article
• Language: English
• Subjects: Animals; Antidepressive Agents - metabolism; Antidepressive Agents - pharmacokinetics; Biological Transport; Depuration; Environmental Exposure - analysis; Fluoxetine; Fluoxetine - analogs & derivatives; Fluoxetine - metabolism; Fluoxetine - pharmacokinetics; Freshwater; Japanese medaka; Norfluoxetine; Oryzias - metabolism; Oryzias latipes; Serotonin Uptake Inhibitors - metabolism; Serotonin Uptake Inhibitors - pharmacokinetics; Uptake
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2008.08.022

The selective serotonin reuptake inhibitor (SSRI) class of anti-depressants is among the most widely prescribed groups of pharmaceuticals. Consequently, aquatic ecosystems impacted by municipal wastewater discharges are predicted to receive substantial annual loadings of these compounds. Although SSRIs have been detected in fish tissues, little is known of their uptake and depuration in freshwater fish species. In this study, Japanese medaka ( Oryzias latipes) were exposed to fluoxetine at a nominal concentration of 0.64 μg L −1 for 7 d and subsequently allowed to depurate in clean water over a 21 d period. Fluoxetine uptake by medaka was observed within the first 5 h of exposure and the biologically active metabolite, norfluoxetine, was also detected in medaka tissues during this timeframe. A maximum fluoxetine concentration was measured in medaka by the third day of the uptake phase, yielding an uptake rate constant ( k 1) of 5.9 ± 0.5 (d −1). During the depuration phase of the experiment, a half life of 9.4 ± 1.1 d was determined for fluoxetine. Using these data, bioconcentration factor (BCF) values of 74 and 80 were estimated for fluoxetine and a pseudo-BCF (the ratio of the concentration of norfluoxetine in medaka and the aqueous fluoxetine concentration) of 117 was calculated for norfluoxetine. These results indicate longer persistence and greater potential for the bioaccumulation of fluoxetine and norfluoxetine in fish tissues than would be predicted from prior half life estimates derived using mammalian species.