Rational design and analysis of an Escherichia coli strain for high-efficiency tryptophan production

l -tryptophan ( l -trp) is a precursor of various bioactive components and has great pharmaceutical interest. However, due to the requirement of several precursors and complex regulation of the pathways involved, the development of an efficient l -trp production strain is challenging. In this study,...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2018-05, Vol.45 (5), p.357-367
Main Authors: Chen, Yuanye, Liu, Yongfei, Ding, Dongqin, Cong, Lina, Zhang, Dawei
Format: Article
Language:English
Subjects:
Acetic acid
Acetyltransferase
Bacteria
Batch culture
Biochemistry
Bioinformatics
Biomedical and Life Sciences
Biotechnology
Combinatorial analysis
Deactivation
E coli
Escherichia coli
Fermentation
Galactose
Genetic Engineering
Genetics and Molecular Biology of Industrial Organisms - Original Paper
Glucokinase
Glucose transport
Inactivation
Inorganic Chemistry
Life Sciences
Microbiology
Permease
Phosphate acetyltransferase
Phosphotransferase
Pyruvate kinase
Pyruvic acid
Tryptophan
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Summary:l -tryptophan ( l -trp) is a precursor of various bioactive components and has great pharmaceutical interest. However, due to the requirement of several precursors and complex regulation of the pathways involved, the development of an efficient l -trp production strain is challenging. In this study, Escherichia coli ( E. coli ) strain KW001 was designed to overexpress the l -trp operator sequences ( trpEDCBA ) and 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase ( aroG fbr ). To further improve the production of l -trp, pyruvate kinase ( pykF ) and the phosphotransferase system HPr ( ptsH ) were deleted after inactivation of repression ( trpR ) and attenuation (attenuator) to produce strain KW006. To overcome the relatively slow growth and to increase the transport rate of glucose, strain KW018 was generated by combinatorial regulation of glucokinase ( galP ) and galactose permease ( glk ) expression. To reduce the production of acetic acid, strain KW023 was created by repressive regulation of phosphate acetyltransferase ( pta ) expression. In conclusion, strain KW023 efficiently produced 39.7 g/L of l -trp with a conversion rate of 16.7% and a productivity of 1.6 g/L/h in a 5 L fed-batch fermentation system.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-018-2020-x