In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide‐induced cytotoxicity and UV‐induced mtDNA damage in human keratinocytes

The traditional practice of eating the flowers of Clitoria ternatea L. or drinking their infusion as herbal tea in some of the Asian countries is believed to promote a younger skin complexion and defend against skin aging. This study was conducted to investigate the protective effect of C. ternatea...

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Bibliographic Details
Published in:Phytotherapy research 2018-06, Vol.32 (6), p.1064-1072
Main Authors: Zakaria, N.N.A., Okello, E.J., Howes, M‐J., Birch‐Machin, M.A., Bowman, A.
Format: Article
Language:English
Subjects:
Aging
Anthocyanins
antioxidant
Clitoria ternatea
Cytotoxicity
Damage
Deoxyribonucleic acid
DNA
DNA damage
Drinking water
Flowers
Glycosides
HaCaT
Herbal tea
Hydrogen
Hydrogen peroxide
Kaempferol
Keratinocytes
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Mitochondrial DNA
Organic chemistry
Oxidative stress
Polymerase chain reaction
Quercetin
Skin
skin aging
Tea
Toxicity
Ultraviolet radiation
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Summary:The traditional practice of eating the flowers of Clitoria ternatea L. or drinking their infusion as herbal tea in some of the Asian countries is believed to promote a younger skin complexion and defend against skin aging. This study was conducted to investigate the protective effect of C. ternatea flower water extract (CTW) against hydrogen peroxide‐induced cytotoxicity and ultraviolet (UV)‐induced mitochondrial DNA (mtDNA) damage in human keratinocytes. The protective effect against hydrogen peroxide‐induced cytotoxicity was determined by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium assay, and mtDNA damage induced by UV was determined by polymerase chain reaction. Preincubation of HaCaT with 100, 250, and 500 μg/ml CTW reduced cytotoxicity effects of H2O2 compared with control (H2O2 alone). CTW also significantly reduced mtDNA damage in UV‐exposed HaCaT (p 
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.6045