Betanin reduces organophosphate induced cytotoxicity in primary hepatocyte via an anti-oxidative and mitochondrial dependent pathway

• Published in: Pesticide biochemistry and physiology 2018-01, Vol.144, p.71-78
• Main Authors: Ahmadian, Elham, Khosroushahi, Ahmad Yari, Eghbal, Mohammad Ali, Eftekhari, Aziz
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Antioxidants - pharmacology; Betacyanins - pharmacology; Betanin; Cell Survival - drug effects; Cells, Cultured; Chlorpyrifos - toxicity; Diazinon - toxicity; Dichlorvos - toxicity; Glutathione - metabolism; Hepatocytes - drug effects; Hepatocytes - enzymology; Hepatocytes - metabolism; L-Lactate Dehydrogenase - metabolism; Lipid Peroxidation; Male; Membrane Potential, Mitochondrial - drug effects; Mitochondria; Mitochondria, Liver - drug effects; Mitochondria, Liver - enzymology; Mitochondria, Liver - metabolism; Organophosphate; Organophosphates - toxicity; Oxidative stress; Pesticides - toxicity; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2017.11.009

Organophosphates (OP) are potent pesticide commonly utilized in agricultural and domestic use. However, plentitude of data represent their side effects in different body tissues. We attempted to study whether betanin (a natural pigment) is able to mitigate some OPs-induced hepatotoxicity in primary rat hepatocytes. Cell viability, lactate dehydrogenase (LDH) leakage, reactive oxygen species (ROS) formation, lipid peroxidation (LPO), glutathione (GSH) depletion and mitochondrial depolarization were tested as toxicity markers. The outcomes revealed that betanin (25μM) significantly increased cell viability, plummeted ROS formation and LPO, restored cellular GSH reservoirs and protected mitochondria after chlorpyrifos (CPF) (300μM), diazinon (DZN) (600μM) and dichlrovos (DDVP) (400μM) treatment. Taken together, all data suggests the potential protective role of betanin in OPs-induced hepatotoxicity in which the mechanism appears to be inhibition of ROS formation and mitochondrial protection. [Display omitted] •Betanin boosts cells viability against organophosphates-induced hepatotoxicity.•Organophosphates-mediated mitochondrial toxicity has been inhibited by betanin.•Betanin has a profound role in mitigation of OP-medaited oxidative damage.