CD3 zeta Expression and T Cell Proliferation are Inhibited by TGF- beta 1 and IL-10 in Cervical Cancer Patients

• Published in: Journal of clinical immunology 2009-07, Vol.29 (4), p.532-544
• Main Authors: Diaz-Benitez, Cinthya E, Navarro-Fuentes, Karla R, Flores-Sosa, Jacqueline A, Juarez-Diaz, Janet, Uribe-Salas, Felipe J, Roman-Basaure, Edgar, Gonzalez-Mena, Ludwig E, Alonso de Ruiz, Patricia, Lopez-Estrada, Guillermina, Lagunas-Martinez, Alfredo, Bermudez-Morales, Victor H, Alcocer-Gonzalez, Juan M, Martinez-Barnetche, Jesus, Hernandez-Pando, Rogelio, Rosenstein, Yvonne, Moreno, Jose, Madrid-Marina, Vicente
• Format: Article
• Language: English
• ISSN: 0271-9142; 1573-2592
• DOI: 10.1007/s10875-009-9279-7

Introduction: Cervical cancer development from a squamous intraepithelial lesion is thought to be favored by an impaired T cell immunity. We evaluated parameters of T cell alterations such as proliferation, cytokine, and CD3 zeta expression in peripheral blood and tumor-infiltrating T lymphocytes from women with squamous intraepithelial lesions (SIL) or cervical cancer (CC). Results and Discussion: T cell proliferation and cytokine messenger RNA (mRNA) expression were similar in women with SIL and healthy donors, whereas low T cell proliferation and lower mRNA expression of IL-2, IL-10 and IFN- gamma were observed in women with CC. Moreover, infiltrating cells showed marginal responses. We also found that CD3 zeta mRNA expression, whose protein is required for T cell activation, correlated with a decreased proliferation in advanced stages of the disease. Conclusion: Experiments with T cells from healthy donors in the presence TGF- beta 1 or IL-10 suggest that these cytokines have a relevant role in T cell responses during CC progression.