Decontamination of aflatoxin duckling feed with aqueous citric acid treatment

• Published in: Animal feed science and technology 2007-06, Vol.135 (3), p.249-262
• Main Authors: Méndez-Albores, A., Del Río-García, J.C., Moreno-Martínez, E.
• Format: Article
• Language: English
• Subjects: acid treatment; Aflatoxin B 1; aflatoxins; Anatidae; animal nutrition; aqueous solutions; Biological and medical sciences; body weight changes; Citric acid; Contaminated feed; decontamination; Detoxification; detoxification (processing); ducklings; experimental diets; Feed and pet food industries; feed contamination; feeds; Food industries; Fundamental and applied biological sciences. Psychology; poultry feeding; Salmonella; toxicity
• ISSN: 0377-8401; 1873-2216
• DOI: 10.1016/j.anifeedsci.2006.07.009

Detoxification of commercial aflatoxin B 1 (AFB 1) contaminated feed by means of 1N aqueous citric acid was confirmed by the AFLATEST™ immunoaffinity column method (IAC), high performance liquid chromatography (HPLC), Ames test ( Salmonella-microsomal screening system), and the 1-day duckling assay. IAC and HPLC determinations showed that AFB 1 at an initial concentration of 110 ng/g was partially detoxified (86%) by the acidic treatment. Results of the Ames test indicated that the mutagenic activity of treated AFB 1 was greatly reduced (or inactivated) compared with that of untreated controls based on his − → his + reversions in the Salmonella TA100 strain. The toxicology and pathology studies showed that toxicity of AFB 1 was minimized by acidic treatment. The results indicated that aqueous citric acid exhibits detoxifying activity in AFB 1-contaminated feeds and protects animals from chronic aflatoxin toxicity.