Targeted and untargeted-metabolite profiling to track the compositional integrity of ginger during processing using digitally-enhanced HPTLC pattern recognition analysis

Tracking the impact of commonly applied post-harvesting and industrial processing practices on the compositional integrity of ginger rhizome was implemented in this work. Untargeted metabolite profiling was performed using digitally-enhanced HPTLC method where the chromatographic fingerprints were e...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2018-03, Vol.1080, p.59-63
Main Authors: Ibrahim, Reham S., Fathy, Hoda
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer - methods
Cluster Analysis
Digitally-enhanced HPTLC
Food Handling
Ginger
Hierarchical clustering analysis
Limit of Detection
Linear Models
Metabolite profiling
Metabolome
Metabolomics - methods
Plant Extracts - analysis
Plant Extracts - metabolism
Plant Extracts - standards
Principal Component Analysis
Reproducibility of Results
Rhizome - chemistry
Rhizome - metabolism
Zingiber officinale - chemistry
Zingiber officinale - metabolism
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Summary:Tracking the impact of commonly applied post-harvesting and industrial processing practices on the compositional integrity of ginger rhizome was implemented in this work. Untargeted metabolite profiling was performed using digitally-enhanced HPTLC method where the chromatographic fingerprints were extracted using ImageJ software then analysed with multivariate Principal Component Analysis (PCA) for pattern recognition. A targeted approach was applied using a new, validated, simple and fast HPTLC image analysis method for simultaneous quantification of the officially recognized markers 6-, 8-, 10-gingerol and 6-shogaol in conjunction with chemometric Hierarchical Clustering Analysis (HCA). The results of both targeted and untargeted metabolite profiling revealed that peeling, drying in addition to storage employed during processing have a great influence on ginger chemo-profile, the different forms of processed ginger shouldn't be used interchangeably. Moreover, it deemed necessary to consider the holistic metabolic profile for comprehensive evaluation of ginger during processing.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2018.02.020