Detection of HBV genome in the plasma and peripheral blood mononuclear cells of Iranian HBsAg negative patients with HIV infection: occult HBV infection

The presence of hepatitis B virus (HBV) DNA in the absence of traceable hepatitis B surface antigen (HBsAg) in the plasma specimen of patients is defined as occult HBV infection (OBI). This study aimed to detect HBV-DNA in the plasma and peripheral blood mononuclear cells (PBMCs) of Iranian HBsAg ne...

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Bibliographic Details
Published in:Archives of virology 2018-06, Vol.163 (6), p.1559-1566
Main Authors: Tajik, Zahra, Bokharaei-Salim, Farah, Ghorbani, Saied, Keyvani, Hossein, Esghaei, Maryam, Monavari, Seyed Hamidreza, Ataei-Pirkooh, Angila, Garshasbi, Saba, Donyavi, Tahereh, Fakhim, Atousa
Format: Article
Language:English
Subjects:
Antiretroviral drugs
Biomedical and Life Sciences
Biomedicine
Deoxyribonucleic acid
DNA
Genomes
Hepatitis
Hepatitis B e antigen
Hepatitis B surface antigen
HIV
Human immunodeficiency virus
Infections
Infectious Diseases
Leukocytes (mononuclear)
Medical Microbiology
Original Article
Peripheral blood mononuclear cells
Plasma
Virology
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Summary:The presence of hepatitis B virus (HBV) DNA in the absence of traceable hepatitis B surface antigen (HBsAg) in the plasma specimen of patients is defined as occult HBV infection (OBI). This study aimed to detect HBV-DNA in the plasma and peripheral blood mononuclear cells (PBMCs) of Iranian HBsAg negative patients with human immunodeficiency virus (HIV) infection. This cross-sectional study was conducted on 172 patients with HIV infection from September 2015 to August 2017. The patients were tested for serological parameters (HBsAg, HBcAb, HBeAg and HBeAb) against HBV infection. Moreover, they were tested for HBV viral load (using COBAS TaqMan 48 Kit, Roche, USA) in plasma and the presence of the HBV genome in PBMC specimens using real-time PCR. The mean age of the patients was 35.4 ± 13.4 years. Of the 172 studied patients, 109 (63.4%) were male. In this study, 151 (87.8%) patients were negative for HBsAg, 111 (64.5%) patients were negative for all HBV infection serological markers, 9 (5.2%) patients were only positive for HBsAg and 29 (16.9%) patients were only positive for HBcAb. Moreover, five (3.3%) patients with HBsAg negative had OBI (in the plasma sample of four patients and PBMC specimens of all five patients, HBV-DNA was detected). The present study revealed that 3.3% of the patients with HIV infection had occult HBV infection. Presumably, designing prospective studies to identify this infection in patients with HIV infection is informative and valuable.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-018-3740-y