Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats

• Published in: Diabetes/metabolism research and reviews 2018-07, Vol.34 (5), p.e2995-n/a
• Main Authors: Shamsaldeen, Yousif A., Ugur, Rosemary, Benham, Christopher D., Lione, Lisa A.
• Format: Article
• Language: English
• Subjects: Animals; Aorta; Carbachol; Caveolin; Caveolin 1 - metabolism; caveolin‐1; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - physiopathology; Dyslipidemia; Dyslipidemias - physiopathology; Endothelial cells; endothelial dysfunction; endothelial nitric oxide synthase; Endothelium, Vascular - metabolism; Endothelium, Vascular - pathology; Hyperglycemia; Hyperglycemia - metabolism; Hyperglycemia - pathology; Low density lipoprotein; Male; Metabolic disorders; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase Type III - metabolism; Nitric-oxide synthase; oxidative LDL; Oxidative Stress; Rats; Rats, Wistar; Rodents; Serum levels; Streptozocin; Vascular Diseases - metabolism; Vascular Diseases - pathology; Vasodilation
• ISSN: 1520-7552; 1520-7560
• DOI: 10.1002/dmrr.2995

Background Diabetes is a complex progressive disease characterized by chronic hyperglycaemia and dyslipidaemia associated with endothelial dysfunction. Oxidized LDL (Ox‐LDL) is elevated in diabetes and may contribute to endothelial dysfunction. The aim of this study was to relate the serum levels of Ox‐LDL with endothelial dysfunction in streptozotocin (STZ)‐diabetic rats and to further explore the changes in endothelial nitric oxide synthase (eNOS) and caveolin‐1 (CAV‐1) expression in primary aortic endothelial cells. Methods Diabetes was induced with a single intraperitoneal injection of STZ in male Wistar rats. During the hyperglycaemic diabetes state serum lipid markers, aortic relaxation and aortic endothelial cell eNOS and CAV‐1 protein expressions were measured. Results Elevated serum Ox‐LDL (STZ 1486 ± 78.1 pg/mL vs control 732.6 ± 160.6 pg/mL, P