Arachidonic acid metabonomics study for understanding therapeutic mechanism of Huo Luo Xiao Ling Dan on rat model of rheumatoid arthritis

Huo Luo Xiao Ling Dan (HLXLD), a traditional Chinese medicine (TCM), is commonly used for the treatment of rheumatoid arthritis (RA). To explore the potential therapeutic mechanism of HLXLD on anti-inflammatory activity. A metabolomic approach based on UFLC–MS/MS to profile arachidonic acid (AA) met...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2018-05, Vol.217, p.205-211
Main Authors: Wang, Nannan, Zhao, Xiaoning, Huai, Jiaxin, Li, Yiran, Cheng, Congcong, Bi, Kaishun, Dai, Ronghua
Format: Article
Language:English
Subjects:
Arachidonic acid
Huo Luo Xiao Ling Dan
Metabonomics
Rheumatoid arthritis
UFLC-MS/MS
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Summary:Huo Luo Xiao Ling Dan (HLXLD), a traditional Chinese medicine (TCM), is commonly used for the treatment of rheumatoid arthritis (RA). To explore the potential therapeutic mechanism of HLXLD on anti-inflammatory activity. A metabolomic approach based on UFLC–MS/MS to profile arachidonic acid (AA) metabolic changes was used. The cyclooxygenase (COX) and lipoxygenase (LOX) catalyzed metabolites in plasma were quantified on 7, 14, 21, and 28 days after the rats injected with Complete Freund's adjuvant and orally administrated with HLXLD, methotrexate and dexamethasone in parallel as the positive control drugs. Nineteen metabolites involved in COX and LOX pathways in RA model group were significant increased compared with normal group (P 
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2018.02.027