Effect of reserpine on Pseudomonas aeruginosa quorum sensing mediated virulence factors and biofilm formation

This study aimed to evaluate the effect of reserpine, a plant-derived indole-alkaloid, against Pseudomonas aeruginosa PAO1 biofilms. The anti-biofilm activity of reserpine was evaluated by crystal violet staining, MTT assay, confocal laser scanning microscopy and scanning electron microscopy. Reserp...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2018-03, Vol.34 (3), p.320-334
Main Authors: Parai, Debaprasad, Banerjee, Malabika, Dey, Pia, Chakraborty, Arindam, Islam, Ekramul, Mukherjee, Samir Kumar
Format: Article
Language:English
Subjects:
Biofilms
Confocal microscopy
DNA
Drug development
Electron microscopy
Gene expression
Indoles
Lasers
Microscopy
Mode of action
Molecular chains
Molecular docking
Molecular modelling
Nucleotide sequence
PCR
Plants
Pseudomonas aeruginosa
Pyocyanin
Quorum sensing
Regulators
Reserpine
Rhamnolipids
Scanning electron microscopy
Scanning microscopy
Transcription
Virulence
Virulence factors
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Summary:This study aimed to evaluate the effect of reserpine, a plant-derived indole-alkaloid, against Pseudomonas aeruginosa PAO1 biofilms. The anti-biofilm activity of reserpine was evaluated by crystal violet staining, MTT assay, confocal laser scanning microscopy and scanning electron microscopy. Reserpine effects were also assessed by qRT-PCR of quorum sensing (QS)-regulated genes and biochemical quantification of the QS-mediated virulence factors pyocyanin, rhamnolipids, proteases and elastases. Reserpine reduced biofilm formation, cell motility, virulence factor production, and QS-controlled gene expression. Additionally, molecular docking analysis for AHL synthase LasI and QS transcriptional regulators LasR/MvfR revealed a plausible molecular mechanisms of reserpine QS inhibition. These findings provide insights into the underlying mode of action of reserpine, which may be useful in the development of new drugs against biofilm-related infections.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2018.1437910