Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen

• Published in: Microbial pathogenesis 2018-04, Vol.117, p.243-246
• Main Authors: Odhah, M.N., Jesse, F.F.A., Lawan, A., Idris, U.H., Marza, A.D., Mahmood, Z.K., Yusuf, A., Arsalan, M., Wahid, A.H., Mohd-Azmi, M.L., Zamri-Saad, M.
• Format: Article
• Language: English
• Subjects: C. pseudotuberculosis; Goats; Haptoglobin; Mycolic acid; Serum amyloid A
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2018.02.038

Haptoglobin (Hp) and Serum Amyloid A (SAA) are a group of blood proteins whose concentrations in animals can be influenced by infection, inflammation, surgical trauma or stress. Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), and Mycolic acid is a virulent factor extracted from C. pseudotuberculosis. There is a dearth of sufficient evidence on the clinical implication of MAs on the responses of Hp and SAA in goats. Therefore, this study was conducted to evaluate the potential effects of Mycolic acid (MAs) and C. pseudotuberculosis on the responses of Hp and SAA in female goats. A total of 12 healthy female goats was divided into three groups; A, B and C each comprising of 4 goats and managed for a period of three months. Group (A) was inoculated with 2 mL of sterile phosphate buffered saline (as a negative control group) intradermally, while group (B) and (C) were inoculated intradermally with 2 ml each of mycolic acid and 1‏ × 109 cfu of active C. pseudotuberculosis respectively. The result of the study showed that the Hp concentration in goats inoculated with C. pseudotuberculosis was significantly increased up to 7-fold (1.17 ± 0.17 ng/L) while MAs showed a 3-fold increased (0.83 ± 0.01 ng/L) compared with the control. Whereas SAA concentration in C. pseudotuberculosis and MAs groups showed a significant 3-fold (17.85 ± 0.91 pg/mL) and 2-fold (10.97 ± 0.71 pg/mL) increased compared with the control. This study concludes that inoculation of C. pseudotuberculosis and MAs have significant effects on Hp and SAA levels, which indicates that MAs could have a role in the pathogenesis of caseous lymphadenitis. •Hp and SAA can be considered as indicators of potential immunogenic responses in challenged goats.•The immunogenic responses are divulged on inoculation with C. pseudotuberculosis and MAs in goats.•The current study also proved that MAs could have an immunogenic attribute.•Hence it does induce an elevated Hp and SAA in the challenged goats.